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Giant cell tumor of bone

Fine structural localization of alkaline phosphatase

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Summary

The fine structural localization of nonspecific alkaline phosphatase was elucidated in two giant cell tumors of bone using lead as capturing ion and Β-glycerophosphate as substrate in the incubation solution. Lead phosphate precipitate — indicating presence of alkaline phosphatase — was demonstrated on the plasma membranes, and the membranes bordering vesicles and vacuoles of presumed endocytotic nature, in giant cells and type 1 stromal cells (fibroblast-like cells). The findings support the view that stromal cells type I and giant cells are histogenetically related.

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References

  • Aparisi, T., Arborgh, B., Ericsson, J.L.E.: Giant cell tumor of bone. An ultrastructural study. Proceedings of the Annual Meeting of the Swedish Medical Association Hygiaea 85, 386 (1976)

    Google Scholar 

  • Aparisi, T., Arborgh, B., Ericsson, J.L.E.: Giant cell tumor of bone. Detailed fine structural analysis of different cell compontns. Virchows Arch. A Path. Anat. and Histol. 376, 273–298 (1977a)

    Google Scholar 

  • Aparisi, T., Arborgh, B., Ericsson, J.L.E.: Giant cell tumor of bone. Fine structural localization of acid phosphatase. Virchows Arch. A Path. Anat. and Histol. 376, 299–308 (1977b)

    Google Scholar 

  • Aparisi, T., Arborgh, B., Ericsson, J.L.E., Göthlin, G., Nilsonne, U.: Contribution to the knowledge of the fine structure of chondrosarcoma. With a note on the localization of alkaline phosphatase and ATPase. Acta Path. Microbiol. Scand. A, Pathology (in press) (1978a)

  • Aparisi, T., Arborgh, B., Ericsson, J.L.E.: Studies on the fine structure of osteoblastoma. With notes on the localization of non specific acid and alkaline phosphatase. Cancer (in press) (1978b)

  • Aparisi, T., Arborgh, B., Ericsson, J.L.E.: Malignant giant cell tumor of bone. Fine structure and localization of acid phosphatase. (Submitted for publication) (1978c)

  • Borgers, M., Thoné, F., De Crée, J., De Cock, W.: Alkaline phosphatase activity in human polymorphonuclear leukocytes. Histochem. J. 10, 31–43 (1978)

    Google Scholar 

  • Gilmer, W.S.: Histochemical studies in bone disease. I. Distribution of acid and alkaline phosphatase in bone tumors as a diagnostid aid. J. Bone Joint Surg. 36 A, 182–183 (1954)

    Google Scholar 

  • Göthlin, G., Ericsson, J.L.E.: Fine structural localization of alkaline phosphatase in the fracture callus of the rat. Histochemie 36, 225–236 (1973)

    Google Scholar 

  • Göthlin, G., Arborgh, B., Ericsson, J.L.E., Helminen, H.: Histochemical and biochemical studies on the localization and activity of lysosomal enzymes in fracture callus. Histochemie 35, 97–110 (1973)

    Google Scholar 

  • Göthlin, G., Ericsson, J.L.E.: The osteoclast. Revue of ultrastructure, origin, and structure-function relationship. Clin. Orthop. 120, 201–231 (1976)

    Google Scholar 

  • Helminen, H., Ericsson, J.L.E.: On the mechanisms of lysosomal enzyme secretion. Electron microscopic and histochemical studies on epithelial cells of the rat's ventral prostate lobe. J. Ultrastruct. Res. 33, 528–549 (1970)

    Google Scholar 

  • Jeffree, G.M., Price, C.H.G.: Bone tumors and their enzymes. J. Bone Joint Surg. 47 B, 120–136 (1965)

    Google Scholar 

  • Kraievski, N.A., Raikhlin, N.T., Soloviov, J.L.: Histochemical characteristics of giant cell bone tumours. Fol. Histochem. Cytochem. 8, 3–10 (1970)

    Google Scholar 

  • Mnaymneh, W., Ghandur-Mnaymneh, L.: Giant cell tumor of bone. Progr. Clin. Cancer 3, 245–280 (1967)

    Google Scholar 

  • Ores, R., Rosen, P., Ortiz, J.: Localization of acid phosphatase activity in a giant cell tumor of bone. Arch. Pathol. 88, 54–57 (1969)

    Google Scholar 

  • Pepler, W.J.: The histochemistry of giant cell tumours (osteoclastoma and giant cell epulis). J. Path. Bact. 76, 505–510 (1958)

    Google Scholar 

  • Schajowicz, F.: Giant cell tumor of bone. An histochemical study. J. Bone Joint Surg. 43 A, 1–29 (1961)

    Google Scholar 

  • Thiery, J.-P., Mazabraud, A., Voisin, M.-C.: Etude cytologique de la tumeur à céllules géantes. Rev. Chir. Orthoped. 61, 377–386 (1975)

    Google Scholar 

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Authors and Affiliations

  1. Department of Orthopedic Surgery, Karolinska Hospital, S-104 01, Stockholm, Sweden

    Tomas Aparisi

  2. Department of Pathology at Huddinge Hospital, Karolinska Institute Medical School, Stockholm

    Bengt Arborgh

  3. Central Laboratory of Clinical Pathology at the University Hospital, University of Uppsala, Sweden

    Jan L. E. Ericsson

Authors
  1. Tomas Aparisi
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  2. Bengt Arborgh
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  3. Jan L. E. Ericsson
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Aparisi, T., Arborgh, B. & Ericsson, J.L.E. Giant cell tumor of bone. Virchows Arch. A Path. Anat. and Histol. 378, 287–295 (1978). https://doi.org/10.1007/BF00465595

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  • DOI: https://doi.org/10.1007/BF00465595

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