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The preservation and regeneration of cilia on human nasal epithelial cells cultured in vitro

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Summary

Dissociated human nasal epithelial cells from nasal polyps were cultured in Ham's F12-DME 1/1 supplemented with NU-serum 10%, choleratoxin (10 ng/ml), retinoic acid (10−7 M) and antibiotics. In monolayer cultures, the epithelial cells grew to confluency on collagen gels, became squamous, and lost their cilia within 2–6 weeks. In suspension cultures, epithelial cell sheaths formed stable vesicles and aggregates. These maintained a respiratory-type morphology and normal ciliary activity for over 6 months. When deciliated, squamous cells from monolayer cultures were brought in suspension, a respiratory-type morphology with cilia reappeared. This in vitro ciliogenesis resulted in normal and coordinated ciliary activity observed for more than 5 months.

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Authors and Affiliations

  1. Center for Human Genetics, University of Leuven, Campus Gasthuisberg O & N6, Herestraat, B-3000, Leuven, Belgium

    M. Jorissen, B. Van der Schueren, H. Van den Berghe & J. -J. Cassiman

  2. ENT Department, University of Leuven, Leuven, Belgium

    M. Jorissen

Authors
  1. M. Jorissen
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  2. B. Van der Schueren
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  3. H. Van den Berghe
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  4. J. -J. Cassiman
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Jorissen, M., Van der Schueren, B., Van den Berghe, H. et al. The preservation and regeneration of cilia on human nasal epithelial cells cultured in vitro. Arch Otorhinolaryngol 246, 308–314 (1989). https://doi.org/10.1007/BF00463582

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