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2-O-methyl-d-xylose containing sheath in the cyanobacterium Gloeothece sp. PCC 6501

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Abstract

The sheath of the unicellular cyanobacterium Gloeothece sp. PCC 6501 was isolated from cell homogenates by differential and sucrose gradient centrifugation, followed by lysozyme treatment and hot sodium dodecyl sulfate extraction. The sheath contains a major fraction of carbohydrate consisting of galactose, glucose, mannose, rhamnose, 2-O-methyl-d-xylose, xylose, glucuronic and galacturonic acids, but only traces of fatty acids and phosphate. A protein content of about 2% (of fraction dry weight) could not be removed by the detergent treatment.

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Abbreviations

SDS:

sodium dodecyl sulfate

References

  • Adhikary SP, Weckesser J, Jürgens UJ, Golecki J, Borowiak D (1986) Isolation and chemical characterization of the sheath from the cyanobacterium Chroococcus minutus SAG B.41.79. J Gen Microbiol 132:2595–2599

    Google Scholar 

  • Drews G, Weckesser J (1982) Fine structure and chemical composition of the cell envelopes. In: Carr NG, Whitton BA (eds) The biology of cyanobacteria. Blackwell Scientific Publications, Oxford, pp 96–116

    Google Scholar 

  • Galambos JT (1967) The reaction of carbazole with carbohydrates. Anal Biochem 19:119–132

    Google Scholar 

  • Golecki JR (1977) Studies on ultrastructure and composition of cell walls of the cyanobacterium Anacystis nidulans. Arch Microbiol 141:35–41

    Google Scholar 

  • Jansson PE, Kenne L, Liedgren H, Lindberg B, Lönngren J (1976) A practical guide to the methylation analysis of carbohydrates. Chem Comm 8:1–74

    Google Scholar 

  • Jürgens UJ, Weckesser J (1985) The fine structure and chemical composition of the cell wall and sheath layers of cyanobacteria. Ann Inst Pasteur/Microbiol 136A:41–44

    Google Scholar 

  • Laidlaw RA (1954) Wood saponins. Part I. A preliminary investigation of the saponins from morabukea {Mora gonggrijpii (Kleinh.) Sandwith}. J Chem Soc (Lond) {1954}:752–757

    Google Scholar 

  • McOmie JFW, Watts ML, West DE (1968) Demethylation of arylmethyl ethers by boron tribromide. Tetrahydron 24:2289–2292

    Google Scholar 

  • Richardson KC, Jarett L, Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Techn 35:313–323

    Google Scholar 

  • Rippka R, Waterbury JB, Cohen-Bazire G (1974) A cyanobacterium which lacks thylakoids. Arch Microbiol 100:419–436

    Google Scholar 

  • Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  • Schopf JW, Walter MR (1982) Origin and early evolution of cyanobacteria: the geological evidence. In: Carr NG, Whitton BA (eds) The biology of cyanobacteria. Blackwell Scientific Publications. Oxford, pp 543–564

    Google Scholar 

  • Schrader M, Drews G, Golecki JR, Weckesser J (1982) Isolation and characterization of the sheath from the cyanobacterium Chlorogloeopsis PCC 6912. J Gen Microbiol 128:267–272

    Google Scholar 

  • Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastructure Res 26:31–43

    Google Scholar 

  • Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171–205

    Google Scholar 

  • Vaara T (1982) The outermost surface structures in chroococcacean cyanobacteria. Can J Microbiol 28:929–941

    Google Scholar 

  • Weckesser J, Drews G, Mayer H (1979) Lipopolysaccharides of photosynthetic prokaryotes. Annu Rev Microbiol 33:215–239

    Google Scholar 

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Weckesser, J., Broll, C., Adhikary, S.P. et al. 2-O-methyl-d-xylose containing sheath in the cyanobacterium Gloeothece sp. PCC 6501. Arch. Microbiol. 147, 300–303 (1987). https://doi.org/10.1007/BF00463492

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  • DOI: https://doi.org/10.1007/BF00463492

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