Abstract
The basis for resistance of yeast form of Histoplasma capsulatum to antifungal activity of human neutrophils was studied. In limiting dilution assays and short term coculture assays human neutrophils were ineffective in killing H. capsulatum whereas Candida albicans was readily killed. By contrast, in a cell free hydrogen peroxide-peroxidase-halide system H. capsulatum was as sensitive to killing as C. albicans. Moreover, lysate of human neutrophils effectively substituted for horse-radish peroxidase in a cell free system for killing H. capsulatum. H. capsulatum elicited significant products of the oxidative burst in human neutrophils as detected by luminol-enhanced chemiluminescence. However, the response was two-fold less (p<0.05) than that induced by C. albicans. Transmission electron microscopy studies showed that phagosome-lysosome fusion took place when neutrophils phagocytosed C. albicans or H. capsulatum. Taken together, these findings indicate that, even though H. capsulatum elicits an oxidative burst and phagosome-lysosome fusion within the phagosome, it is capable of evading damage in short term assays.
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Abbreviations
- CFU:
-
colony forming units
- PMN:
-
polymorphonuclear neutrophil
- CTCM:
-
complete tissue culture medium
- CL:
-
chemiluminescence
- HPO:
-
horseradish peroxidase
- P-L:
-
lysosomal peroxidase positive material
References
DeMonbreum WA. The cultivation and cultural characteristic of Darlings Histoplasma capsulatum. Am J Trop Med 1934; 14: 93–125.
Ajello L. Coccidioidomycosis and histoplasmosis: a review of their epidemiology and geographical distribution. Mycopathol Mycol Appl 1971; 45: 221–30.
Schwartz J. The pathogenesis of histoplasmosis. Trans NY Acad Sci 1958; 20: 541–48.
Schaffner A, Davis CE, Schaffner T, Market M, Douglas H, Braude AI. In vitro susceptibility of fungi to killing by neutrophil granulocytes discriminates between primary pathogenicity and opportunism. J Clin Invest 1986; 78: 511–24.
Reiss E, Miller SE, Kaplan W, Kauffman L. Antigenic, chemical, and structural properties of cell walls of Histoplasma capsulatum yeast-form chemotype 1 and 2 after serial enzymatic hydrolysis. Infect Immun 1977; 16: 690–700.
Brummer E, Stevens DA. Opposite effects of human monocytes, macrophages, and polymorphonuclear neutrophils on replication of Blastomyces dermatitidis in vitro. Infect Immun 1982; 36: 297–303.
Sugar AM, Chahal RS, Brummer E, Stevens DA. Susceptibility of Blastomyces dermatitidis strains to products of the oxidative metabolism. Infect Immun 1983; 41: 908–12.
Brummer E, McEwen JG, Stevens DA. Fungicidal activity of murine inflammatory polymorphonuclear neutrophils: comparison with murine peripheral blood PMN. Clin Exp Immunol 1986; 66: 681–90.
Allen RC, Loose LD. Phagocytic activation of luminol dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochem Biophys Res Comm 1976; 69: 245–52.
Bertram TA, Canning PC, Roth JA. Preferential inhibition of primary granule release from bovine neutrophils by a Brucella abortus extract. Infect Immun 1986; 52: 285–292.
Wells MY, Rikihisa Y. Lack of lysosomal fusion with phagosomes containing Ehrlichia risticn in P388D1 cells: abrogation of inhibition with oxytetracycline. Infect Immun 1988; 56: 3209–3215.
Howard DA. Fate of Histoplasma capsulatum in guinea pig polymorphonuclear leukocytes. Infect Immun 1973; 8: 412–19.
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Kurita, N., Terao, K., Brummer, E. et al. Resistance of Histoplasma capsulatum to killing by human neutrophils. Mycopathologia 115, 207–213 (1991). https://doi.org/10.1007/BF00462229
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DOI: https://doi.org/10.1007/BF00462229