Abstract
Cytochromes b, c, d, and o were identified by spectroscopic analysis of respiratory membrane fragments from Vitreoscilla sp., strain C1. Carbon monoxide difference spectra of the reduced membranes had absorption maxima at 416, 534, and 571 nm (ascribed to cytochrome o) and 632 nm (cytochrome d). Derivative spectra of the pyridine hemochromogen spectra of the membranes identified the presence of b- and c-type cytochromes in Vitreoscilla. The cyanide binding curve of the membranes was biphasic with dissociation constants of 2.14 mM and 10.7 mM which were assigned to cytochrome o and cytochrome d, respectively. Membranes bound carbon monoxide with dissociation constant 3.9 μM, which was assigned to cytochrome o. Cytochrome c 556 and a NADH-p-iodonitrotetrazolium violet reductase component were partially purified from Vitreoscilla membranes.
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Abbreviations
- INT:
-
p-iodonitrotetrazolium violet
- RMF:
-
respiratory membrane fragments
- K d :
-
dissociation constant
- CHAPS:
-
3-[(3-cholamido propyl) dimethylammonio]-1-propanesulfonate
- DOC:
-
sodium deoxycholate
- PAGE:
-
polyacrylamide gel electrophoresis
- SDS:
-
sodium dodecyl sulfate
References
DeMaio RA, Webster DA (1983) Presence of two different cytochromes o in Vitreoscilla. Fed Proc 42:1843
Dulley JR, Grieve PA (1975) A simple technique for eliminating interference by detergents in the Lowry method of protein determination. Anal Biochem 64:136–141
Ensley BD, Finnerty WR (1980) Influences of growth substrates and oxygen on the electron transport system in Acinetobacter sp. H01-N. J Bacteriol 142:859–868
Fuhrhop JH, Smith KM (1975) Porphyrins and metalloporphyrins. Smith KM (e). North-Holland Biomedical Press, Amsterdam, pp 804–807
Georgiou CD, Webster DA (1987) Purification and partial characterization of the membrane-bound cytochrome o (561, 564) from Vitreoscilla. Biochemistry, accepted for publication (in press)
Gonzalez-Prevatt V, Webster DA (1980) Purification and properties of NADH-cytochrome o reductase from Vitreoscilla. J Biol Chem 255:1478–1482
Green GN, Gennis RB (1983) Isolation and characterization of an Escherichia coli mutant lacking cytochrome d terminal oxidase. J Bacteriol 154:1269–1275
Hackett NR, Bragg PD (1983a) Membrane cytochromes of Escherichia coli grown aerobically and anaerobically with nitrate. J Bacteriol 154:708–718
Hackett NR, Bragg PD (1983b) Membrane cytochromes of Escherichia coli chl mutants. J Bacteriol 154:719–727
Kaback HR (1971) Bacterial membranes. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol XXII. Academic Press, New York, pp 99–120
Kita K, Konishi K, Anraku Y (1984a) Terminal oxidases of Escherichia coli aerobic respiratory chain. I. Purification and properties of cytochrome b 562-o complex from cells in the early exponential phase of aerobic growth. J Biol Chem 259:3368–3374
Kita K, Konishi K, Anraku Y (1984b) Terminal oxidases of Escherichia coli aerobic respiratory chain. II. Purification and properties of cytochrome b 558-d complex from cells grown with limited oxygen and evidence of branched electron-carrying systems. J Biol Chem 259:3375–3381
Koland JG, Miller MJ, Gennis RB (1984) Potentiometric analysis of the purified cytochrome d terminal oxidase complex from Escherichia coli. Biochemistry 23:1051–1056
Kranz RG, Gennis RB (1983) Immunological characterization of the cytochrome o terminal oxidase from Escherichia coli. J Biol Chem 258:10614–10621
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T. Nature 227:680–685
Meyer TE, Kamen DM (1982) New perspective on c-type cytochromes. In: Anfinsen CB, Edsall JT, Richards FM (eds) Advances in protein chemistry, vol 35. Academic Press, New York, pp 105–212
Miller JM, Gennis RB (1983) The purification and characterization of the cytochrome d terminal oxidase complex of the Escherichia coli aerobic respiratory chain. J Biol Chem 258:9159–9165
Nicols P, Stulp BK, Jones JG, White DC (1986) Comparison of fatty acid content and DNA homology of the filamentous gliding bacteria Vitreoscilla, Flexibacter, Filibacter. Arch Microbiol 146:1–6
Poole RK (1983) Bacterial cytochrome oxidases: a structurally and functionally diverse group of electron-transfer proteins. Biochim Biophys Acta 726:205–243
Poole RK, Waring AJ, Chance B (1979) The reaction of cytochrome o in Escherichia coli with oxygen. Biochem J 184:379–389
Reichenbach H, Ludwig W, Stackebrandt E (1986) Lack of relationship between gliding cyanobacteria and filamentous gliding heterotrophic eubacteria: comparison of 16S rRNA catalogues of Spirulina, Saprospira, Vitreoscilla, Leucothrix, and Herpetosiphon. Arch Microbiol 145:391–395
Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175:720–731
Strohl WR, Schmidt TM, Lawry NH, Mezzino MJ, Larkin JM (1986a) Characterization of Vitreoscilla beggiatoides and Vitreoscilla filiformis sp. nov., nom. rev., and comparison with Vitreoscilla stercoraria and Beggiatoa alba. Internat J Syst Bacteriol 36:302–313
Strohl WR, Schmidt TM, Vinci VA, Larkin JM (1986b) Electron transport and respiration in Beggiatoa and Vitreoscilla. Arch Microbiol 145:71–75
Tzangoloff A, Penefsky HS (1971) Extraction, and purification of lipoprotein complexes from membranes. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol XXII. Academic Press, New York, pp 219–230
Wakabayashi S, Matusbara H, Webster DA (1986) Primary sequence of a dimeric bacterial hemoglobin from Vitreoscilla. Nature 322:481–483
Webster DA, Liu CY (1974) Reduced nicotinamide adenine dinucleotide cytochrome o reductase associated with cytochrome o purified from Vitreoscilla. J Biol Chem 249:4257–4260
Wilson DF (1967) Effect of temperature on the spectral properties of some ferrocytochromes. Arch Biochem Biophys 121:757–768
Woese CR, Weisburg WG, Paster BJ, Hahn CM, Tanner RS, Krieg NR, Koops H-P, Harms H, Stackebrandt E (1984) The phylogeny of purple bacteria: the beta subdivision. System Appl Microbiol 5:327–336
Yamanaka T, Okunuki T (1974) Cytochromes. In: Neilands JB (ed) Microbial iron metabolism. Academic Press, New York, pp 349–400
Yamazaki T, Fukumori Y, Yamanaka T (1985) Cytochrome a 1 of Nitrosomonas europaea resembles aa 3-type cytochrome c oxidase in many respects. Biochim Biophys Acta 810:174–183
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Georgiou, C.D., Webster, D.A. Identification of b, c, and d cytochromes in the membrane of Vitreoscilla . Arch. Microbiol. 148, 328–333 (1987). https://doi.org/10.1007/BF00456712
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DOI: https://doi.org/10.1007/BF00456712