Abstract
Export of porin to the outer membrane of the phototrophic purple bacterium Rhodobacter capsulatus was studied with the use of the uncoupler of the electron transport chain, carbonylcyanide-m-chlorophenylhydrazone (CCCP). The agent reversibly blocked the transport of porin across the cytoplasmic membrane. By means of radioactive labeling and immunoprecipitation, porin was found to occur in two forms: (i) the exported form that was extractable from the outer membrane without disrupting the cells, and (ii) a pre-form with a slightly higher apparent molecular mass which accumulated in the cells during the block of the export process. Proteolysis studies revealed that the preform was highly sensitive to added proteases, whereas the exported form was resistant.
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Abbreviations
- CCCP:
-
carbonylcyanide-m-chlorophenylhydrazone
- DMSO:
-
dimethylsulfoxide
- EDTA:
-
ethylenediamine tetraacetic acid
- OMP:
-
outer membrane porin; pre-OMP, form of outer membrane porin before export
- PAGE:
-
polyacrylamide gel electrophoresis
- PMSF:
-
phenylmethylsulfonyl fluoride
- SDS:
-
sodium dodecyl sulfate
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Woitzik, D., Weckesser, J. & Dierstein, R. Export of porin to the outer membrane of the phototrophic bacterium Rhodobacter capsulatus 37B4. Arch. Microbiol. 152, 196–200 (1989). https://doi.org/10.1007/BF00456101
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DOI: https://doi.org/10.1007/BF00456101