Abstract
Synthesis of threonine dehydratase in Streptomyces fradiae was positively influenced by valine and negatively by isoleucine. However, these two amino acids had no effect on the activity of this enzyme. Synthesis of threonine dehydratase in α-aminobutyrate resistant mutants of S. fradiae was pronouncedly less sensitive to the positive effect of valine and this change in regulation led to valine overproduction. Synthesis of acetohydroxy acid synthase is regulated in a similar manner to that of threonine dehydratase, however a lower level of expression was detected in α-aminobutyrate resistant mutants. And again, no effect of branched-chain amino acids on acetohydroxy acid synthase activity was observed. It follows that in S. fradiae synthesis of threonine dehydratase is the main regulatory mechanism governing production and the mutual ratio of synthesized valine and isoleucine.
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Abbreviations
- α-AB:
-
α-aminobutyrate
- AHAS:
-
acetohydroxy acid synthase
- α-KB:
-
α-ketobutyrate
- MNNG:
-
N-methyl-N′-nitro-N-nitrosoguanidine
- TD:
-
threonine dehydratase
- Trans. B.:
-
transaminase of branched-chain amino acids
- VDH:
-
valine dehydrogenase
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Vančura, A., Vančurová, I., Kopecký, J. et al. Regulation of branched-chain amino acid biosynthesis in Streptomyces fradiae, a producer of tylosin. Arch. Microbiol. 151, 537–540 (1989). https://doi.org/10.1007/BF00454871
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DOI: https://doi.org/10.1007/BF00454871