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The influence of indomethacin on biomechanical and biochemical properties of the plantaris longus tendon in the rabbit

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Summary

It has previously been reported that indomethacin inhibits fracture healing and heterotopic bone formation. Stimulated by these reports, we undertook the present investigation to study the influence of indomethacin on biomechanical and biochemical properties of the plantaris longus tendon in the rabbit. Sixty-eight New Zealand White rabbits were used for the experiment. Half of them were treated with indomethacin, 10 mg/kg orally a day, and the other half with placebo. After 4, 8, and 16 weeks of treatment biomechanical and biochemical variables were determined and compared between the two groups. After 16 weeks there was a significant increase in tensile strength in the group treated with indomethacin. There was no certain concomitant change in the total collagen content, the amounts of soluble and insoluble collagen, or the water content. Further investigations concerning the influence of indomethacin on tendon healing are indicated.

Zusammenfassung

Es gibt verschiedene Mitteilungen über die Hemmung von Frakturheilung und von heterotropischer Knochenbildung durch Indomethacin. Angeregt von solchen Berichten haben wir die Wirkung von Indomethacin auf die biomechanischen und biochemischen Eigenschaften der Plantaris longus-Sehne von Kaninchen untersucht. Insgesamt wurden 68 New Zealand White-Kaninchen als Versuchstiere benutzt, wovon die eine Hälfte täglich 10 mg/kg Indomethacin per os bekam and die andere Hälfte Placebo. Nach 4, 8 and 16 Behandlungswochen wurden biomechanische and biochemische Parameter untersucht and zwischen den beiden Gruppen verglichen. Nach 16 Wochen fanden wir einen signifikanten Anstieg der Zugfestigkeit nach Indomethacin-Behandlung. Keine sichere Veränderung des Gesamtgehaltes an Kollagen - löslichem und unlöslichem — oder des Gehaltes an Wasser wurde registriert. Weitere Untersuchungen über den Einfluß von Indomethacin auf die Sehnenheilung erscheinen uns notwendig.

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References

  1. Almåsbakk K, Röysland P (1977) Does indomethacin (IMC) prevent postoperative ectopic ossification in total hip replacement (Abstract)? Acta Orthop Scand 48:556

    Google Scholar 

  2. Butler DL, Grood ES, Noyes FR (1978) Biomechanics of ligaments and tendons. Exerc Sport Sci Rev 6:125–181

    Google Scholar 

  3. Carlstedt CA, Skagervall R (1986) A model for computeraided analysis of biomechanical properties of the plantaris longus tendon in the rabbit. J Biomech 19:251–256

    Google Scholar 

  4. Elves MW, Bayley I, Röylance PJ (1982) The effect of Indomethacin upon experimental fractures in the rat. Acta Orthop Scand 53:35–41

    Google Scholar 

  5. McIntosh JEA, McIntosh RP (1980) Mathematical modelling and computers in endocrinology. Monographs on Endocrinology, vol 16. Springer, Berlin Heidelberg New York

    Google Scholar 

  6. Piez KA (1968) Cross-linking of collagen and elastin. Ann Rev Biochem 37:547–570

    Google Scholar 

  7. Rø J, Langeland N, Sander J (1978) Effect of Indomethacin on collagen metabolism of rat fracture callus in vitro. Acta Orthop Scand 49:323–328

    Google Scholar 

  8. Rø J, Sudmann E, Marton PF (1978) Effect of Indomethacin on fracture healing in rats. Acta Orthop Scand 47:588–599

    Google Scholar 

  9. Samuelsson B (1974) Endogenous synthesis of prostaglandins in guinea pig and man: effects of inhibitors. In: Robinson HJ, Vane JR (eds) Prostaglandin synthetase inhibitors. Raven Press, New York, pp 99–106

    Google Scholar 

  10. Stegemann H, Stalder KH (1967) Determination of hydroxyproline. Clin Chim Acta 18:267–273

    Google Scholar 

  11. Sudmann E (1975) Effect of indomethacin on bone remodelling in rabbit ear chambers. Acta Orthop Scand [Suppl] 160:91–115

    Google Scholar 

  12. Sudmann E, Bang G (1979) Indomethacin-induced inhibition of haversian remodelling in rabbits. Acta Orthop Scand 50:621–627

    Google Scholar 

  13. Sudmann E, Dregelid E, Bessesen A, Møland J (1979) Inhibition of fracture healing by indomethacin in rats. Eur J Clin Invest 9:333–339

    Google Scholar 

  14. Sudmann E, Hagen T (1976) Indomethacin-induced delayed fracture healing. Arch Orthop Unfallchir 85:151–154

    Google Scholar 

  15. Sudmann E, Tveita T, Hald J Jr (1982) Lack of effect of Indomethacin on ordered growth of the femur in rats. Acta Orthop Scand 53:43–49

    Google Scholar 

  16. Viidik A (1968) Elasticity and tensile strength of the anterior cruciate ligament in rabbits as influenced by training. Acta Physiol Scand 74:372–380

    Google Scholar 

  17. Viidik A, Danielsen CC, Oxlund H (1982) On fundamental and phenomenological models, structure and mechanical properties of collagen elastin and glycosaminoglycan complexes. Biorheology 19:437–451

    Google Scholar 

  18. Vogel HC (1977) Mechanical and chemical properties of various connective tissue organs in rats as influenced by non-steroidal antirheumatic drugs. Connect Tiss Res 5:91–95

    Google Scholar 

  19. Vogel HC (1978) Influence of maturation and age on mechanical and biochemical parameters of connective tissue of various organs in the rat. Connect Tiss Res 6:161–166

    Google Scholar 

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Author notes

  1. C. A. Carlstedt

    Present address: Hospital for Joint Diseases, Orthopaedic Institute, 301 East Seventeenth Street, 10003, New York, NY, USA

Authors and Affiliations

  1. Karolinska Institute, Department of Orthopaedic Surgery, Huddinge University Hospital, Huddinge, Sweden

    C. A. Carlstedt & T. Wredmark

  2. Department of Histology, Karolinska Institute, Stockholm, Sweden

    K. Madsen

Authors
  1. C. A. Carlstedt
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  2. K. Madsen
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  3. T. Wredmark
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Carlstedt, C.A., Madsen, K. & Wredmark, T. The influence of indomethacin on biomechanical and biochemical properties of the plantaris longus tendon in the rabbit. Arch. Orth. Traum. Surg. 106, 157–160 (1987). https://doi.org/10.1007/BF00452202

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  • DOI: https://doi.org/10.1007/BF00452202

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