Summary
Eighty-eight rats underwent intramedullary pin fixation and fracture of both tibiae. Half of the animals were given clodronate 50 mg/kg s. c. weekly. Clodronate treatment did not affect the growth of fibrocartilage or the endochondral and membranous new bone formation. The regaining of tensile load capacity of fractured bone remained unaffected by the drug. Calluses were remodeled to lamellar bone in both groups. However, although the total area invaded by mineralized tissue in callus remained unaffected by the drug, the areas of hematopoietic bone marrow tissue within mineralized callus were observed to be markedly smaller in clodronate-treated animals than in controls. The calluses in the clodronate group were significantly heavier and contained more calcium at 2 months after fracture than those in the controls.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bauer HCF, Törnqvist H, Nilsson OS (1986) Effects of biphosphonates on the incorporation of calcium-45 and 3H-proline it orthotopic and in demineralized matrix-induced bone in rats. Bone 7:129
Chappard D, Petitjean M, Alexandre C, Vico L, Minaire P, Riffat G (1991) Cortical osteoclasts are less sensitive to etidronate than trabecular osteoclasts. J Bone Joint Res 6:673–680
Flanagan AM, Chambers TJ (1989) Dichloromethylenebiphosphonate (Cl2MBP) inhibits bone resorption through injury to osteoclasts that resorb Cl2MBP-coated bone. Bone Miner 6:33
Frost HM (1989) The biology of fracture healing. An overview for clinicians. I and II. Clin Orthop 248:283
Goldner J (1938) A modification of the Masson trichrome technique for routine laboratory purposes. Am J Pathol 14:237
Järvinen M (1976) Healing of a crush injury in rat striated muscle. Acta Chir Scand 142:47
Jung A, Bornand J, Mermillod B, Edouard C, Meunier PI (1984) Inhibition by diphosphonates of bone resorption induced by the Walker tumor of the rat. Cancer Res 44:3007
Miller SC, Jee WSS (1979) The effect of dichloromethylene diphosphonate, a pyrophospate analog, on bone and bone cell structure in the growing rat. Anat Rec 193:439
Mönkkönen J (1988) A one-year follow-up study of the distribution of 14C-clodronate in mice and rats. Pharmacol Toxicol 62:51
Mönkkönen J, Ylitalo P (1990) The tissue distribution of clodronate (dichloromethylene biphosphonate) in mice. The effects of vehicle and the route of administration. Eur J Drug Metab Pharmacokinet 3:239
Penttinen R, Niinikoski J, Kulonen E (1972) Hyperbaric oxygenation and fracture healing. A biochemical study with rats. Acta Chir Scand 138:147
Schenk P, Eggli P, Fleisch H, Rosini S (1986) Quantitative morphometric evaluation of the inhibitory activity of new aminobiphosphonates on bone resorption in the rat. Calcif Tissue Int 38:342
Vaes G (1988) Cellular biology and biomechanical mechanism of bone resorption. A review of recent developments on the formation, activation and mode of action of osteoclasts. Clin Orthop Rel Res 231:239
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nyman, M.T., Paavolainen, P. & Lindholm, T.S. Clodronate increases the calcium content in fracture callus. Arch Orthop Trauma Surg 112, 228–231 (1993). https://doi.org/10.1007/BF00451880
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00451880