Abstract
Pure cultures of methanogenic bacteria were shown to utilize acetate as a methanogenic substrate. In the presence of hydrogen, both Methanosarcina barkeri and Methanobacterium thermoautotrophicum rapidly converted acetate to methane. This reaction was shown to be dependent on the concentration of hydrogen and acetate. In the absence of hydrogen, acetate was not fermented by methane bacteria. Both the methyl and carboxyl position of acetate were reduced to methane. More 14C-methane was detected from methyl than carboxyl-labeled acetate. The utilization of acetate by cultures of M. thermoautotrophicum was enhanced by addition of CO2/HCO3 −. Methyl labeled acetate was shown to be incorporated into whole cells and converted to 14CO2 and 14CH4 in the presence of H2 and 25 mM CO2/HCO3 −. The importance of acetate utilization in microbial methanogenesis was discussed.
Similar content being viewed by others
References
Bryant, M. P., Robinson, I. M.: An improved non-selective culture medium for ruminal bacteria and its use in determining diurnal variation in number of bacteria in the rumen. J. Dairy Sci. 44, 1446–1456 (1961)
Cappenberg, Th. E., Prins, R. A.: Interrelations between sulfate-reducing and methane producing bacteria in bottom deposits of a fresh-water lake. III. Experiments with 14C-labeled substrates. Antonie v. Leeuwenhoek 40, 456–469 (1974)
Chen, R. L., Keeney, D. R., Konrad, J. G., Holding, A. J., Graetz, D. A.: Gas metabolism in sediments of Lake Mendota. J. Environ. Quanlity 1, 155–156 (1972)
Decker, K., Jungermann, K., Thauer, R. K.: Energy production in anaerobic organisms. Angew. Chem. Internat. Edit. 9, 138–158 (1970)
Hungate, R. E.: The anaerobic mesophilic cellulolytic bacteria. Bact. Rev. 14, 1–49 (1950)
Jeris, J. S., McCarty, P. L.: The biochemistry of methane fermentation using C14 tracers. J. Water Poll. Control Fed. 37, 178–192 (1965)
Kirsch, E. J., Sikes, R. M.: Anaerobic digestion in biological waste treatment. Progr. Ind. Microbiol. 9, 155–239 (1971)
Lawrence, A. W., McCarty, P. L.: Kinetics of methane fermentation in anaerobic treatment. J. Water Poll. Control Fed. 41, R1-R17 (1969)
McCarty, P. L.: Thermodynamics of biological synthesis and growth. In: Adv. Water Poll. Res., p. 169–186. New York: Pergamon Press 1975
Nelson, D. R.: Studies on microbial methanogenesis in Lake Mendota. Dissertation, University of Wisconsin, Madison, Wi. (1974)
Nelson, D. R., Zeikus, J. G.: Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism. Appl. Microbiol. 28, 258–261 (1974)
Pine, M. J., Barker, H. A.: Studies on the methane fermentation. XII. The pathway of hydrogen in acetate fermentation. J. Bact. 71, 644–648 (1956)
Pretorius, W. A.: The effect of formate on the growth of acetate utilizing methanogenic bacteria. Water Res. 6, 1213–1217 (1972)
Smith, P. H., Mah, R. A.: Kinetics of acetate metabolism during sludge digestion. Appl. Microbiol. 14, 368–371 (1966)
Stadtman, T. C., Barker, H. A.: Studies on the methane fermentation. VII. Tracer studies. Arch. Biochem. 21, 256–264 (1949)
Stadtman, T. C., Barker, H. A.: Studies on the methane fermentation. IX. The origin of methane in the acetate and methanol fermentations by Methanosarcina. J. Bact. 61, 81–86 (1951)
Toerien, D. E., Thiel, P. G., Pretorius, W. A.: Substrate flow in anaerobic digestion. Adv. Water Poll. Res. 11, 2911–2917 (1971)
Wolfe, R. S.: Microbial formation of methane, p. 107–146. In: A. H. Rose, J. F. Wilkinson, eds., Adv. microbial physiol., Vol. 6. New York-London: Academic Press 1971)
Wolin, E. A., Wolin, M. J., Wolfe, R. S.: Formation of methane by bacterial extracts. J. biol. Chem. 238, 2882–2886 (1963)
Zeikus, J. G., Ward, J. G.: Methane formation in living trees: a microbial origin. Science 184, 1181–1183 (1974)
Zeikus, J. G., Wolfe, R. S.: Methanobacterium thermoautotrophicum sp. n., an anaerobic, autotrophic, extreme thermophile. J. Bact. 109, 707–713 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zeikus, J.G., Weimer, P.J., Nelson, D.R. et al. Bacterial methanogenesis: Acetate as a methane precursor in pure culture. Arch. Microbiol. 104, 129–134 (1975). https://doi.org/10.1007/BF00447312
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00447312