Abstract
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1.
ATP-Dependent phosphorylation of fructose could not be detected in extracts of fructose-grown cells of Pseudomonas extorquens strain 16, Pseudomonas 3A2, Pseudomonas acidovorans and Pseudomonas fluorescens. Instead, phosphorylation of fructose to fructose-1-phosphate was found to occur when cell-free extracts were incubated with fructose and phosphoenolpyruvate. Such an activity could not be detected in cell-free extracts of succinate-grown cells.
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2.
High levels of 1-phosphofructokinase were found in extracts of the above organisms when grown on fructose.
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3.
Mutants of Pseudomonas extorquens strain 16 lacking 1-phosphofructokinase were unable to grow on fructose. Revertants to growth on fructose had regained the capacity to synthesize this enzyme, indicating its necessary involvement in fructose metabolism.
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4.
A survey has been carried out of enzymes involved in carbohydrate metabolism in the species listed above.
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Abbreviations
- PEP-fructose-PTS:
-
phosphoenol-pyruvate-fructose-phosphotransferase
- G-6-P:
-
glucose-6-phosphate
- FDP:
-
fructose-1,6-diphosphate
- F-1-P:
-
fructose-1-phosphate
- 1-PFK:
-
1-phosphofructokinase
- 6-PG:
-
6-phosphogluconate
- PEP:
-
phosphoenolpyruvate
- F-6-P:
-
fructose-6-phosphate
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Van Dijken, J.P., Quayle, J.R. Fructose metabolism in four Pseudomonas species. Arch. Microbiol. 114, 281–286 (1977). https://doi.org/10.1007/BF00446874
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DOI: https://doi.org/10.1007/BF00446874