Abstract
Cell-free extracts of d-fructose grown cells of Pseudomonas putida, P. fluorescens, P. aeruginosa, P. stutzeri, P. mendocina, P. acidovorans and P. maltophila catalyzed a P-enolpyruvate-dependent phosphorylation of d-fructose and contained 1-P-fructokinase activity suggesting that in these species fructuse-1-P and fructose-1,6-P2 were intermediates of d-fructose catabolism. Neither the 1-P-fructokinase nor the activity catalyzing a P-enolpyruvate-dependent phosphorylation of d-fructose was present in significant amounts in succinate-grown cells indicating that both activities were inducible. Cell-free extracts also contained activities of fructose-1,6-P2 aldolase, fructose-1,6-P2 phosphatase, and P-hexose isomerase which could convert fructose-1,6-P2 to intermediates of either the Embden-Meyerhof pathway or Entner-Doudoroff pathway. Radiolabeling experiments with 1-14C-d-fructose suggested that in P. putida, P. aeruginosa, P. stutzeri, and P. acidovorans most of the alanine was made via the Entner-Doudoroff pathway with a minor portion being made via the Embden-meyerhof pathway. An edd - mutant of P. putida which lacked a functional Entner-Doudoroff pathway but was able to grow on d-fructose appeared to make alanine solely via the Embden-Meyerhof pathway.
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Abbreviations
- cpm:
-
counts per min
- edd - :
-
mutant lacking Entner-Doudoroff dehydrase (6-PGA dehydrase)
- EDP:
-
Entner-Doudoroff pathway
- EMP:
-
Embden-Meyerhof pathway
- FDP:
-
fructose-1,6-P2
- FDPase:
-
FDP phosphatase
- F-1-P:
-
fructose-1-P
- F-6-P:
-
fructose-6-P
- FPTs:
-
PEP: d-fructose phosphotransferase system
- G-6-P:
-
glucose-6-P
- KDPG:
-
2-keto-3-deoxy-6-P-gluconate
- PEP:
-
P-enolpyruvate
- 1-PFK:
-
1-P-fructokinase
- 6-PFK:
-
6-P-fructokinase
- 6-PGA:
-
6-P-gluconate
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Sawyer, M.H., Baumann, P., Baumann, L. et al. Pathways of d-fructose catabolism in species of Pseudomonas . Arch. Microbiol. 112, 49–55 (1977). https://doi.org/10.1007/BF00446653
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DOI: https://doi.org/10.1007/BF00446653