Summary
Adenine-requiring mutant strains of S. pombe enter the stationary phase after depleting a culture medium of adenine or its analogues. Stationary phase cells of six mutants defective at different stages of the purine nucleotide synthetic pathway were examined for cell volume and DNA content, and then compared in these respects with those of a prototrophic wild-type strain. The cell cycle of the wild-type strain was arrested in the G2 phase (2C state) in the nitrogen rich medium, as is evident from DNA content per cell (0.0425 pg) and cell volume (47.7 μm3). An AIR carboxylase-deficient (ade6) mutant strain was found to have an unusual cell volume (307.4 μm3) and DNA content (0.1187 pg). By DAPI fluorescence microscopy, each mutant cell was seen to contain only one enlarged nucleus, which indicates the absence of cell populations containing cells in the 4C state of the S phase following nuclear division. It then follows that in ade6 mutant cells, DNA synthesis occurs in the absence of a completed nuclear division. Thus in S. pombe cells, the completion of nuclear division is not necessarily required for the next cycle initiation of DNA synthesis under certain physiological conditions.
Abbreviations
- AIR :
-
aminoimidazole ribonucleotide
- DAPI :
-
4′,6-diamidino-2-phenylindole
- PCA :
-
perchloric acid
- DABA :
-
3,5-diaminobenzoic acid
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Ishiguro, J. An abnormal cell division cycle in an AIR carboxylase-deficient mutant of the fission yeast Schizosaccharomyces pombe . Curr Genet 15, 71–74 (1989). https://doi.org/10.1007/BF00445754
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DOI: https://doi.org/10.1007/BF00445754