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Aspergillus growth and aflatoxin production on black pepper

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Abstract

Black peppercorns supported the growth of Aspergillus parasiticus (flavus) NRRL 3145. Incubation at 28 C and RH of 85% for 30 days resulted in profuse conidial production on the reticulate ridges of the peppercorns. An SEM study of these ridges showed that they were cortical eruptions, and the particulate matter present favored mycelial establishment. Hyphal anastamoses were commonly observed. Following this incubation the inoculated peppercorns were stored in the dark at room temperature and ambient humidity for 15 days. Aflatoxin was detected on a minicolumn. Aflatoxin B1 was produced at levels of 60 μg/kg with traces of aflatoxins B 2, G1 and G2. Quantitative estimation was based on a visual comparison with standards by thin-layer chromatography.

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  1. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada

    M. Seenappa & A. G. Kempton

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  1. M. Seenappa
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  2. A. G. Kempton
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Seenappa, M., Kempton, A.G. Aspergillus growth and aflatoxin production on black pepper. Mycopathologia 70, 135–137 (1980). https://doi.org/10.1007/BF00443023

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