Summary
Prophage λ is not induced when DNA synthesis ceases at 42°C in a mutant of E. coli which is unable to initiate rounds of DNA replication at high temperature. However, induction occurs when the cells are UV irradiated after completion of rounds of replication at 42°C. Evidence is presented that the uvr functions, necessary for dimer excision, are not required for this induction, and that the UV irradiation itself does not provoke net host DNA synthesis under these conditions. We conclude that prophage induction can result from irradiation damage in chromosomes that are unable to replicate.
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Monk, M., Gross, J. Induction of prophage λ in a mutant of E. coli K12 defective in initiation of DNA replication at high temperature. Molec. Gen. Genet. 110, 299–306 (1971). https://doi.org/10.1007/BF00438272
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DOI: https://doi.org/10.1007/BF00438272