Abstract
Nine strains of Pythium insidiosum the etiologic agent of pythiosis, were inoculated on 2% water agar plus grass blades and then incubated one day at 25°C, 35°C and 37°C. Sporangium and secondary biflagellate-type zoosporas from the parasitized grass blades were noticed in induction medium after one hour of incubation at 35 °C and 37 °C. The number of sporangia and zoospores were lower at 25 °C, than 35 °C and 37 °C. Increasing the days of incubation of the parasitized grass blades resulted in the increase in the time of incubation in the induction medium. Corn meal agar, Schmitthenner medium and Sabouraud dextrose agar were also tested but the sporangium and zoosporas were always observed after five hours of incubation in induction medium.
Similar content being viewed by others
References
Amemiya J, Nishiyama S. Isolation of a fungus of Mortierellaceae from a equine granular dermatitis I. Bull Fac Agric Kagoshima 1966; 17:215–24.
Austwick PKC, Copland JW. Swamp cancer. Nature 1974; 250:84.
Bridges CH, Emmons CE. A phycomycosis of horse caused by Hyphomyces destruens. J Am Vet Med Assoc 1961; 138:579–89.
de Cock WAE, Mendoza L, Padhye AA, Ajello L, Kaufman L. Pythium insidiosum sp. nov. The etiologic agent of pythiosis. J Clin Microbiol 1987; 25:344–49.
de Haan J. Bosartige Schimmelkrankheit der Pferdes (Hyphomycosis destruens equi). Zentralbl. Bakt Parasitenkd Infektioskr Hyg Abt l Orig 1902; 31:758–63.
de Haan J, Hoogkamer LJ. Hyphomycosis destruen-equi. Arch Wis Prakt Tierheilk 1903; 29:395–410.
dosSantos MN, Londero AT. Zigomicosis subcutanea em cavalos Pesquisas Agropecuarias Brasileira 1974; 9:7–8.
Endo RM, Colt WM. Anatomy, citology and physiology of infection by Pythium. Procc Am Phytopathol Soc 1974; 1:215–23.
English PB, Frost AJ. Phycomycosis in a dog. Aust Vet J 1984; 61:291–92.
Fothergill PG, Hide D. Comparative nutricional studies of Pythium spp J gen Microbiol 1962; 29:325–34.
Fuller MS. Lower Fungi in the Laboratory. Dept Botany, University of Georgia, Athens USA 1978; pp. 99–100.
Gonzalez HE, Ruiz A. Espundia equina. Etiologia y patogenesis de una ficomicosis. Rev ICA (Colombia) 1975; 10:175–85.
Hickman CJ, Ho HH. Behavior of zoospores in plantpathogenic phycomycetes Annu Rev Phytopathol 1966; 4:195–220.
Kendrick B. The fifth kingdom. Mycologue Publications. Waterloo, Canada 1985; pp. 9–19.
Mendoza L, Alfaro AA. Equine pythiosis in Costa Rica. Report of 39 cases. Mycopathologia 1986; 94:123–29.
Middlenton JT. The taxonomic, host range, and geographic distribution of the genus Pythium. Mem. Torrey Bot. Club 1943; 20:1–171.
Miller RI, Quails CW, Turnwald GH. Gastrointestinal phycomycosis in a dog. J Am Vet Med Assoc 1983; 182:1245–46.
Miller RI, Bruce MO, Archer M. Cutaneous pythiosis in beef calves. J Am Vet Med Asocc 1986; 186:984–86.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mendoza, L., Prendas, J. A method to obtain rapid zoosporogenesis of Pythium insidiosum . Mycopathologia 104, 59–62 (1988). https://doi.org/10.1007/BF00437925
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00437925