Summary
The circular plasmid pKD1 (or 1.6 μm DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 μ DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology. pKD1 can be transferred to Kluyveromyces lactis where it is replicated stably. Using recombinant molecules derived from pKDl, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 μ-based Saccharomyces cerevisiae transformation system.
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Bianchi, M.M., Falcone, C., Re, C.X. et al. Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 μm circular plasmid pKD1. Curr Genet 12, 185–192 (1987). https://doi.org/10.1007/BF00436877
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DOI: https://doi.org/10.1007/BF00436877