Abstract
Agaritine (A), an ingredient of the cultivated mushroom of commerce Agaricus bisporus, was administered by subcutaneous injection to two groups of randomly bred Swiss mice. In the first group the animals of both sexes were treated at a 100 μg/g body weight basis five times at weekly intervals, while in the second group the mice received a single A treatment of 100 μg/g body weight for females and 50 μg/g body weight for males. The administration of the compound resulted in no detectable carcinogenic effect in the animals. Since some of the breakdown products of A were shown to be carcinogenic in mice and the mushroom itself was found to be mutagenic, the field is discussed in the light of the obtained results.
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Daniels, E. G., Kelly, R. B. & Hinman, J. W., 1961. Agaritine: an improved isolation procedure and confirmation of structure by synthesis. J. Am. Chem. Soc. 83: 3333–3334.
Lawson, T. & Toth, B., 1983. Metabolic conversion of arylhydrazines of the cultivated mushroom Agaricus bisporus. Proc. Am. Assoc. Cancer Res. 24: 77.
Levenberg, B., 1961. Structure and enzymatic cleavage of agaritine, a phenylhydrazide of L-glutamic acid isolated from Agaricaceae. J. Am. Chem. Soc. 83: 503–504.
Rogan, E. G., Walker, B. A., Gingell, R., Nagel, D. & Toth, B., 1982. Microbial mutagenicity of selected hydrazines. Mut. Res. 102: 413–419.
Ross, A. E., Nagel, D. & Toth, B., 1982. Evidence for the occurrence and formation of diazonium ions in the Agaricus bisporus mushroom and its extracts. J. Agr. Food Chem. 30: 521–525.
Ross, A., Nagel, D. & Toth, B., 1982. Occurrence, stability and decomposition of β-N[γ-L(+)glutamyl]-4-hydroxyme-thylphenylhydrazine (agaritine) of the mushroom Agaricus bisporus. Food Chem. Toxicol. 20: 903–907.
Sterner, O., Bergman, R., Kesler, E., Magnusson, G., Wickberg, B. & Zimerson, E., 1982. Mutagens in larger fungi. I. Forty-eight species screened for mutagenic activity in the Salmonella/microsome assay. Mut. Res. 101: 269–281.
Toth, B., Rappaport, H. & Shubik, P., 1963. The influence of dose and age on the induction of malignant lymphomas and other tumors by 7,12-dimethylbenz(a)anthracene in Swiss mice. J. Natl. Cancer Inst. 30: 723–741.
Toth, B. & Wilson, R. B., 1971. Blood vessel tumorigenesis by 1,2-dimethylhydrazine dihydrochloride (symmetrical). Gross, light and electron microscopic descriptions. I. Am. J. Pathol. 64: 585–600.
Toth, B., 1972. A toxicity method with calcium cyclamate for chronic carcinogenesis experiments. Tumori 58: 137–142.
Toth, B. & Shimizu, H., 1974. 1-Carbamyl-2-phenylhydrazine tumorigenesis in Swiss mice. Morphology of lung adenomas. J. Natl. Cancer Inst. 52: 241–251.
Toth, B., Nagel, D., Patil, K., Erickson, J. & Antonson, K., 1978. Tumor induction with the N'-acetyl derivative of 4-hydroxymethylphenylhydrazine, a metabolite of agaritine of Agaricus bisporus. Cancer Res. 38: 177–180.
Toth, B., Patil, K., Erickson, J. & Kupper, R., 1979. False morel mushroom Gyromitra esculenta toxin: N-methyl-N-formylhydrazine carcinogenesis in mice. Mycopathologia 68: 121–128.
Toth, B., Patil, K. & Jae, H. S., 1981. Carcinogenesis of 4-(hydroxymethyl)benzenediazonium ion (tetrafluoroborate) of Agaricus bisporus. Cancer Res. 41: 2444–2449.
Toth, B., Raha, C. R., Wallcave, L. & Nagel, D., 1981. Attempted tumor induction with agaritine in mice. Anticancer Res. 1: 255–258.
Toth, B., Nagel, D. & Ross, A., 1982. Gastric tumorigenesis by a single dose of 4-(hydroxymethyl)benzenediazonium ion of Agaricus bisporus. Brit. J. Cancer 46: 417–422.
U.S. Department of Agriculture, Statistical Reporting Service, 1982. Crop Reporting Board. Mushrooms, Washington, D.C., Vg 2-1-2:1–4.
U.S. Department of Agriculture, Foreign Agricultural Service, 1982. Fresh and processed vegetables, Washington, D.C., FVEG 1–82.
Wallcave, L., Nagel, D., Raha, C. R., Jae, H. S., Bronczyk, S., Kupper, R. & Toth, B., 1979. An improved synthesis of agaritine. J. Org. Chem. 44: 3752–3755.
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Toth, B., Sornson, H. Lack of carcinogenicity of agaritine by subcutaneous administration in mice. Mycopathologia 85, 75–79 (1984). https://doi.org/10.1007/BF00436706
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DOI: https://doi.org/10.1007/BF00436706