Skip to main content
Log in

Enhancement of biological treatment performance of saline wastewater by halophilic bacteria

  • Originals
  • Published:
Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Performances of biological treatment processes of saline wastewater are usually low because of adverse effects of salt on microbial flora. High salt concentrations in wastewater cause plasmolysis and loss of activity of cells resulting in low COD removal efficiencies. In order to improve biological treatment performance of saline wastewater, a halophilic organism Halobacter halobium was used along with activated sludge culture.

A synthetic wastewater composed of diluted molasses, urea, KH2PO4 and various concentrations of salt (1%–5% NaCl) was treated in an aerobic-biological reactor by fed-batch operation. Activated sludge culture with and without Halobacter were used as seed cultures. Variations of COD removal rate and efficiency with salt concentration were determined for both cultures and results were compared. Inclusion of Halobacter into activated sludge culture resulted in significant improvements in COD removal efficiency. A rate expression including salt inhibition effect was proposed and kinetic constants were determined by using experimental data.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ingram, M.: The influence of sodium chloride and temperature on the endogenous respiration of Bacillus cercus. J. Gen. Physiol. 23 (1940) 773

    Google Scholar 

  2. Doudoroff, M.: Experiments on the adaptation of E. coli to sodium chloride. J. Gen. Physiol. 23 (1940) 585

    Google Scholar 

  3. Kincannon, D.F.; Gaudy, A.F.: Some effects of high salt concentration on activated sludge. J. WPCF. 38 (1966) 1148–1158

    Google Scholar 

  4. Kincannon, D.F.; Gaudy, A.F.: Response of biological waste treatment systems to changes in salt concentrations. Biotechnol. Bioeng. 10 (1968) 483–496

    Google Scholar 

  5. Burnett, W.E.: The effect of salinity variations on the activated sludge process. Wat. Sew. Works 121 (1974) 37–38

    Google Scholar 

  6. Oren, A.; Gurevich, P.; Malkit, A.; Henis, Y.: Microbial degradation of pollutants at high salt concentrations. Biodegradation. 3 (1992) 387–398

    Google Scholar 

  7. Lawton, G.W.; Eggert, C.V.: Effect of high sodium chloride concentration on trickling filter slimes. J. Wat. Pollut. Cont. Fed. 29 (1957) 1228–1236

    Google Scholar 

  8. Stewart, M.J.; Ludwig, H.F.; Kearns, W.H.: Effects of varying salinity on the extended aeration process. J. Wat. Poll. Con. Fed. 34 (1962) 1161–1177

    Google Scholar 

  9. Ludzack, F.J.; Noran, D.K.: Tolerance of high salinities by conventional wastewater treatment processes. J. Wat. Poll. Cont. Fed. 37 (1965) 1404–1416

    Google Scholar 

  10. Kinner, N.E.; Bishop, P.L.; Asce, M.: Treatment of saline domestic wastewater using RBC's. J. Environ. Eng., ASCE. 108 (1962) 650–663

    Google Scholar 

  11. Belkin, S.; Brenner, A.; Abeliovich, A.: Biological treatment of a high salinity chemical industrial wastewater. Wat. Sci. Technol. 27 (1993) 105–112

    Google Scholar 

  12. Woolard, C.R.; Irvine, R.L.: Treatment of hypersaline wastewater in the sequencing batch reactor. Wat. Res. 29 (1995) 1159–1168

    Google Scholar 

  13. Woolard, C.R.; Irvine, R.L.: Biological treatment of hypersaline wastewater by a biofilm of halophilic bacteria. Wat. Env. Res. 66 (1994) 230–235

    Google Scholar 

  14. Shuler, M.L.; Kargi, F.: Bioprocess Engineering: Basic Concepts. Prentice Hall, USA (1992)

    Google Scholar 

  15. Pirt, S.J.: Principles of Microbe and Cell Cultivation, Blackwell Scientific, England (1975)

    Google Scholar 

  16. Standard Methods for the Examination of Water and Wastewater. 17th Edn. (1989), APHA. Washington, D.C.

Download references

Author information

Authors and Affiliations

Authors

Additional information

This study was supported by the Technical and Scientific Research Council of Turkey.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kargi, F., Dinçer, A.R. Enhancement of biological treatment performance of saline wastewater by halophilic bacteria. Bioprocess Engineering 15, 51–58 (1996). https://doi.org/10.1007/BF00435529

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00435529

Keywords

Navigation