Summary
Viable hybrids of Saccharomyces cerevisiae were obtained by transfer of isolated diploid nuclei into haploid protoplasts using a polyethylene glycol (PEG) fusion procedure.
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Becher, D., Conrad, B. & Böttcher, F. Genetic transfer mediated by isolated nuclei in Saccharomyces cerevisiae . Curr Genet 6, 163–165 (1982). https://doi.org/10.1007/BF00435217
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DOI: https://doi.org/10.1007/BF00435217