Summary
Chloroplast DNA was isolated from total cellular DNA of a bleached mutant of Euglena gracilis (Y3BUD) by enrichment of the light component (p = 1.686) by repeated CsCl equilibrium centrifugations. Electron microscope visualization of this DNA showed minicircular DNA molecules in addition to large circular molecules (42 pm) identical to wild type chloroplast DNA. They were heterogenous in size and their contour lengths ranged from 0.8 to 8.5 μm. Fractionnation by agarose gel electrophoresis gave several discrete bands. Some of them hybridized with pure chloroplast DNA and with several cloned chloroplast DNA fragments, particularly to ribosomal fragments, while others did not show homology with chloroplast DNA being probably of extrachloroplatic origin.
Similar content being viewed by others
References
Chelm BK, Hoben PJ, Hallick RB (1977) Biochem 16:782–785
Chu NM, Oishi KK, Tewari KK (1981) Plasmid 6:279–292
Davis RW, Simon M, Davidson N (1971) Meth Enzymol 21:413–428
Freyssinet G, Heizmann P, Verdier G, Trabuchet G, Nigon V (1970) Physiol Veg 10:421–442
Gelvin SB, Howell SH (1979) Mol Gen Genet 173:315–322
Gibson WH, Hershberger CL (1975) Arch Biochem Biophys 168: 8–14
Hallick RB (1982) In: The Biology of Euglena, vol. 4 Buetow DE (ed) (in press)
Heizmann P, Doly J, Hussein Y, Nicolas P, Nigon V, Bernadi G (1981) Biochim Biophys Acta 653:412–415
Koller B, Delius H (1982) FEBS Letters 139:86–92
Kolodner R, Tewari KK (1975) J Biol Chem 250:8840–8847
Kopecka H, Crouse EJ, Stutz E (1977) Eur J Biochem 72:525–535
Manning JE, Richards OC (1972) Biochem 11:2036–2043
Mielenz JR, Hershberger CL (1974) Biochem Biophys Res Comm 58:769–777
Nass MMK, Ben-Shaul Y (1972) Biochim Biophys Acta 272:130–136
Rawson JRY, Boerma C (1976) Proc Natl Acad Sci USA 73: 2401–2404
Richards OC, Manning JE (1975) In: Lefort-Tran M, Valencia R, (eds) Les Cycles Cellulaires. Editions du CNRS, pp 212–221
Rochaix JD, Malnoe P (1978) Cell 15:661–670
Schiff JA, Epstein HT (1965) In: Locke M (ed) Reproduction: Molecular, Subcellular, Cellular. Academic Press, New York, pp 131–189
Stutz E, Vandrey JP (1971) Proc. IInd Intern. Congr; on Photosynth. Forti G, Avron M, Melandri A (eds) pp 2601–2609
Stutz E, Crouse EJ, Graf L (1975) In: Les Cycles Cellulaires, pp 255–265
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Heizmann, P., Ravel-Chapuis, P. & Nigon, V. Minicircular DNA having sequence homologies with chloroplast DNA in a bleached mutant of Euglena gracilis . Curr Genet 6, 119–122 (1982). https://doi.org/10.1007/BF00435210
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00435210