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Time-schedule dependency of the inhibiting activity of various anticancer drugs in the clonogenic assay

  • Original Articles
  • Anticancer Drugs, Clonogenic Assay, Chemosensitivity, Time Dependency
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Summary

To analyze the discrepancy between the in vitro response in the clonogenic assay and the clinical response, the time-schedule dependencies of various anticancer drugs were determined by comparing the inhibiting effect against colony formation by PC-7 cells treated with the drugs for 1 h with that of those treated for 24h. According to their schedule dependency the drugs can be divided into a schedule-dependent drug group (5-fluorouracil, methotrexate, bleomycin, pepleomycin, etoposide, cisplatin, teniposide, vindesine, and vinblastine) and a non-schedule-dependent drug group (adriamycin, actinomycin D, ranomustine, mitomycin C, aclacinomycin, daunomycin, nimustine, melphalan, and KW 2083). In the clonogenic assay, the 1-h exposure schedule is appropriate for predicting clinical response for the non-schedule-dependent drugs. However, the effect of the schedule-dependent drugs was underestimated in the same conditions. Therefore, it is necessary to test these drugs in the assay by 24-h exposure for a more accurate assessment of their antitumor activity.

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Authors and Affiliations

  1. Pharmacology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, 104, Tokyo, Japan

    Yuka Matsushima, Fumihiko Kanzawa & Akio Hoshi

  2. Department of Internal Medicine, National Cancer Center Hospital, 1-1, Tsukiji 5-chome, Chuo-ku, 104, Tokyo, Japan

    Eiji Shimizu, Hiroaki Nomori, Yasutsuna Sasaki & Nagahiro Saijo

Authors
  1. Yuka Matsushima
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  2. Fumihiko Kanzawa
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  3. Akio Hoshi
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  4. Eiji Shimizu
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  5. Hiroaki Nomori
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  6. Yasutsuna Sasaki
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  7. Nagahiro Saijo
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Matsushima, Y., Kanzawa, F., Hoshi, A. et al. Time-schedule dependency of the inhibiting activity of various anticancer drugs in the clonogenic assay. Cancer Chemother. Pharmacol. 14, 104–107 (1985). https://doi.org/10.1007/BF00434345

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  • DOI: https://doi.org/10.1007/BF00434345

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