Summary
A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.
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Hinchliffe, E., Box, W.G. Expression of the cloned endo-1,3-1,4-β-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae . Curr Genet 8, 471–475 (1984). https://doi.org/10.1007/BF00433914
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DOI: https://doi.org/10.1007/BF00433914