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Isolation and characterization of yeast mutants auxotrophic for 2′-deoxythymidine 5′-monophosphate

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Summary

Mutant strains of Saccharomyces cerevisiae auxotrophic for deoxythymidine monophosphate (dTMP) were isolated and characterized. Two distinct classes of auxotrophs were obtained. One class had a simple requirement for dTMP and was analogous to thymine-requiring bacteria. The second class required dTMP, adenine, histidine and methionine and this complex nutritional phenotype was due to defects in folate metabolism. The dTMP-dependent growth of respiratory-competent grande auxotrophs was found to be markedly affected by media composition and carbon source. In the absence of dTMP thymineless death occurred in both mutant classes.

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Authors and Affiliations

  1. Department of Biology, York University, M3J 1P3, Toronto, Canada

    J. G. Little & R. H. Haynes

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  1. J. G. Little
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  2. R. H. Haynes
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Communicated by G. O'Donovan

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Little, J.G., Haynes, R.H. Isolation and characterization of yeast mutants auxotrophic for 2′-deoxythymidine 5′-monophosphate. Molec. Gen. Genet. 168, 141–151 (1979). https://doi.org/10.1007/BF00431440

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  • DOI: https://doi.org/10.1007/BF00431440

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