Summary
Neoplastic and non-neoplastic tissue specimens from ten patients with primary adenocarcinoma of the urinary bladder were examined. Most of these tumors were associated with either foci of transitional cell carcinoma and/or with glandular metaplasia of the bladder epithelium. The mucin produced by the neoplastic cells was PAS, alcian blue, mucicarmine, PB/KOH/PAS, and RPB/KOH/PAS-positive. ABH isoantigens of these tumors were not always deleted. Ultrastructurally, the neoplastic cells resembled goblet cells. Their plasma membrane had numerous microvilli with prominent glycocalyx. Proliferation and attenuation of tight junctions were noted. The gap junctions were few and small. Two types of desmosomes were found. The ultrastructural features of the neoplastic cells were attributed in part to the malignant transformation and in part to the direction of their differentiation. We have not observed any distinctive morphologic, histochemical, immunologic or ultrastructural features that might be diagnostic for these adenocarcinomas.
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Abdelfattah-Gad M, Denk H (1980) Epithelial blood group antigens in human carcinomas of the distal colon: Further studies on their pathologic significance. J Natl Cancer Inst 64:1025–1029
Alroy J (1979) Tight junctions adjacent to tumor stromal interface in human invasive transitional cell carcinomas. Virchows Arch [Cell Pathol] 30:289–296
Alroy J, Banner BF, Pauli BU, Leav I (1978) Alterations of intercellular junctions in acinic cell carcinoma of the canine pancreas. Virchows Arch [Cell Pathol] 28:21–30
Alroy J, Gould VE (1980) Epithelial stromal interface in normal and neoplastic human bladder epithelium. Ultrastruct Pathol 1:203–212
Alroy J, Pauli BU, Hayden JE, Gould VE (1979) Intracytoplasmic lumina in bladder carcinomas. Hum Pathol 10:549–555
Alroy J, Pauli BU, Weinstein RS (1981) Correlation between desmosome ultrastructure and tumor aggressiveness in human urinary bladder transitional cell carcinoma. Cancer 47:104–112
Alroy J, Pauli BU, Weinstein RS, Merk FB (1977) Association of asymmetric unit membrane plaque formation in the urinary bladder of adult humans with therapeutic radiation. Experientia 33:1645–1647
Alroy I, Teramura K, Miller AW, Pauli BU, Gottesman JE, Flannagan M, Davidsohn I, Weinstein RS (1978) Isoantigens A, B and H in urinary bladder carcinomas following radiotherapy. Cancer 41:1739–1745
Alroy J, Teramura K, Weinstein RS, Davidsohn I (1978) A method for demonstrating blood group isoantigens in permanent tissue section. Stain Technol 53:53–56
Alroy J, Weinstein RS (1976) Unusual cell junction complex in canine mammary gland adenocathomas. J Natl Cancer Inst 56:667–670
Austin GE, Safford J (1978) Signet ring cell carcinoma of bladder. Urology 12:458–460
Coon JS, IV, Weinstein RS (1981) Variability in the expression of O “H” antigen in human transitional epithelium. J Urol 125:301–306
Culling CFA, Reid PE, Burton JD, Dunn WL (1975) A histochemical method of differentiating lower gastrointestinal tract mucin from other mucins in primary of metastatic tumors. J Clin Pathol 28:656–658
Cummings KB (1980) Carcinoma of the bladder: Predictors. Cancer 45:1849–1855
Cunha GR, Chung LWK, Shannon JM, Reese BA (1980) Stromal epithelial interaction in sex differentiation. Biol Reprod 22:19–42
Daroca PJ Jr, McKenzie F, Reed RJ, Keane J (1976) Primary adenovillous carcinoma of the bladder. J Urol 115:41–45
Davidsohn I, Kovarik S, Lee CL (1966) A, B and O substances in gastrointestinal carcinoma. Arch Pathol 81:381–390
Deture FA, Dein R, Hackett RL, Drylee DM (1975) Primary signet ring cell carcinoma of bladder exemplifying vesical epithelial multipotentiality. Urology 6:240–244
Elias PM, Friend DS (1976) Vitamin A induced mucous metaplasia: An in vitro system for modulating tight and gap junction differentiation. J Cell Biol 68:173–188
Fenoglio CM, Ferenczy A, Richart RM (1975) Mucinous tumors of the ovary. Ultrastructural studies of mucinous cystadenomas with histogenetic considerations. Cancer 36:1709–1722
Fulker MJ, Cooper EH, Tanaka T (1971) Proliferation and ultrastructure of papillary transitional cell carcinoma of the human bladder. Cancer 27:71–82
Fuselier HA Jr, Brannan W, Ochsner MG, Matos LH (1978) Adenocarcinoma of the bladder as seen at Ochsner medical institutions. South Med J 71:804–806
Headington JH (1977) Primary mucinous carcinoma of skin. Histochemistry and electron microscopy. Cancer 39:1055–1063
Hickey WF, Seiler MW (1981) Ultrastructural markers of colonic adenocarcinoma. Cancer 47:140–145
Hicks RM (1976) Changes in differentiation of urinary bladder during denign and neoplastic hyperplasia. In: Müller-Berat, Rosenfeld, Tarin and Viza (eds) Progress in Differentiation Research. North-Holland Publ. Co., Amsterdam, pp 339–353
Hicks RM, Wakefield JSJ (1976) Membrane changes during urothelial hyperplasia and neoplasia. Cancer Res 36:2502–2507
Jacob J, Ludgate CM, Ford J, Tulloch WS (1978) Recent observations on the ultrastructure of human urothelium. 1. Normal bladder of elderly subjects. Cell Tissue Res 193:543–560
Jacobo E, Loenig S, Schmidt JD, Culp DA (1977) Primary adenocarcinoma of the bladder: A retrospective study of 20 patients. J Urol 117:54–56
Jacobs JB, Arai M, Cohen SM, Fridell GH (1976) Early lesions in experimental bladder cancer: Scanning electron microscopy of cell surface markers. Cancer Res 36:2512–2517
Jewett HJ (1973) Cancer of the bladder: Diagnosis and staging. Cancer 32:1072–1074
Jones WA, Gibbons RP, Correa RJ Jr, Cummings KB, Manson JT (1980) Primary adenocarcinoma of bladder. Urology 15:119–122
Kjaergaard J, Starklint H, Bierring F, Thybo E (1977) Surface topography of the healthy and diseased transitional cell epithelium of the human urinary bladder. Urol Int 32:34–38
Koss GL (1979) Mapping of the urinary bladder: Its impact on the concepts of bladder cancer. Hum Pathol 10:533–548
Kramer SA, Bredeal J, Croker BP, Paulson DF, Glenn JF (1979) Primary non-urechal adenocarcinoma of the bladder. J Urol 121:278–281
Kunze E (1979) Development of urinary bladder cancer in the rat. Cur Top Pathol 67:147–232
McNutt NS, Weinstein RS (1973) Membrane ultrastructure at mammalian intercellular junctions. Progr Biophys Mol Biol 26:45–101
Melicow MM (1955) Tumors of the urinary bladder: A clinicopathological analysis of over 2500 specimens and biopsies. J Urol 74:498–521
Merk FB, Leav I, Kwan PWL, Ofner P (1980) Effects of estrogen and androgen on the ultrastructure of secretory granules and intercellular junctions in regressed canine prostate. Anat Rec 197:111–132
Merk FB, Pauli BU, Jacobs JB, Alroy J, Friedell GH, Weinstein RS (1977) Malignant transformation of urinary bladder in humans and in N-(4-(5-nitro-2-furyl)-2-thiazolyl) formamide-exposed Fischer rats. Ultrastructure of major components of the permeability barrier. Cancer res 37:2843–2853
Monis B, Dorfman HD (1967) Some histochemical observations on transitional epithelium of man. J Histochem Cytochem 15:475–481
Moor M, Mühlethaller K (1963) Fine structure in frozen-etched yeast cells. J Cell Biol 71:609–628
Mostofi FK (1975) Pathology of malignant tumors of urinary bladder. In: Cooper, and Williams (eds) The Biology and Clinical Management of Bladder Cancer. Blackwell Scientific Publications, Oxford, pp 87–109
Mostofi FK, Thomson RV, Dean AI Jr (1955) Mucous adenocarcinomas of the urinary bladder. Cancer 8:741–758
Nevalainen TJ, Jarvi OH (1977) Ultrastructure of intestinal and diffuse type gastric carcinoma. J Pathol 122:129–136
Panukcu AM (1974) Tumors of the urinary bladder. Bull WHO 50:43–52
Parsons CG, Stauffer C, Schmidt JD (1980) Bladder-surface glycosaminoglycans: An efficient methanism of environmental adaptation. Science, 208:605–607
Prutkin L (1975) Mucous metaplasia and gap junctions in the Vitamin A acid treated skin tumor, keratoacanthoma. Cancer Res 35:364–369
Pund ER, Yount AH, Blumberg JM (1952) Variations in morphology of urinary bladder epithelium. Special references to cystitis glandularis and carcinomas. J Urol 68:242–251
Shrom SH, Parsons CL, Mulholland SG (1977) Role of urothelial surface mucoprotein in intrinsic bladder defense. Urology 9:526–533
Spjut HJ, Smith MN (1967) A comparative electron microscopic study of human and rat colonic polyps and carcinomas. Exp Mol Pathol 6:11–24
Steinbrecher JS, Silverberg SC (1976) Signet ring cell carcinoma of the breast. The mucinous variant of infiltrating carcinoma. Cancer 37:838–840
Szulman AE (1960) The histological distribution of blood group substances A and B in man. J Exp Med 111:785–800
Weinstein RS, Merk FB, Alroy J (1976) The structure and function of intercellular junctions in cancer. Adv Cancer Res 23:23–89
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This study was supported by the Institute of International Education, New York; the Charlton Fund, Tufts University School of Medicine; and Grants#CA-15776 and CA 23776 from the National Cancer Institute
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Alroy, J., Roganovic, D., Banner, B.F. et al. Primary adenocarcinomas of the human urinary bladder: Histochemical, immunological and ultrastructural studies. Virchows Arch. A Path. Anat. and Histol. 393, 165–181 (1981). https://doi.org/10.1007/BF00431074
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DOI: https://doi.org/10.1007/BF00431074