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Proliferation kinetics of malignant non-Hodgkin's lymphomas related to histopathology of lymph node biopsies

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Summary

Since lymphomas of high and low malignancy have been differentiated by the Kiel-classification, the cell kinetics of malignant lymphomas have become an interesting subject. In 20 non-Hodgkin's lymphomas we have determined the initial labelling index, the mitosis index, the proliferation rate and the durations of DNA-synthesis and mitosis. The kinetic variables have been estimated by using an in vitro-incubation of fresh biopsies and double-labelling with radioactive DNA-precursors.

In 8 lymphomas of low malignancy we found an initial labelling index of 7.8%, a mitosis index of 0.3% and a potential tumour doubling time of 100.2 h for a cell production rate without regard to cell loss. On the other hand, lymphomas of high malignancy showed a labelling index of 16.7%, a mitosis index of 0.7% and a potential tumour doubling time of 40.5 h. All these values could be differentiated with high statistical confidence. DNA-synthesis time and mitosis time of both lymphoma groups did not show any significant differences.

From these findings we obtained confirmation of the cyto-morphological principles used in classifying lymphomas.

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Authors and Affiliations

  1. Institute of Pathology, Medical School Hannover, Karl-Wiechert-Allee 9, D-3000, Hannover 61, Germany

    W. Lang & S. Kienzle

  2. Department of Onkology, Medical School Hannover, Karl-Wiechert-Allee 9, D-3000, Hannover 61, Germany

    V. Diehl

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  1. W. Lang
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  2. S. Kienzle
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  3. V. Diehl
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Supported by the Deutsche Forschungsgemeinschaft (DFG, grant Ge 121/16)

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Lang, W., Kienzle, S. & Diehl, V. Proliferation kinetics of malignant non-Hodgkin's lymphomas related to histopathology of lymph node biopsies. Virchows Arch. A Path. Anat. and Histol. 389, 397–407 (1980). https://doi.org/10.1007/BF00430662

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