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The human leukocyte test system

VI. The use of sister chromatid exchanges as possible indicators for mutagenic activities

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Summary

The trifunctional alkylating chemical mutagen trenimon increases the frequency of sister chromatid exchanges in human leukocyte chromosomes in vitro, as revealed by a BUdR-Giemsa method. Treatment with lead acetate exhibited negative results in this respect. The use of sister chromatid exchanges as possible indicators for mutagenic activities is discussed.

Zusammenfassung

Das trifunktionelle alkylierende Chemomutage Trenimon erhöht die Häufigkeit von Schwesterchromatiden-Austauschen in menschlichen Leukocytenchromosomen in vitro. Dies wird anhand einer BUdR-Giemsa-Methode gezeigt. Die Behandlung mit Bleiacetat liefert in dieser Hinsicht negative Ergebnisse. Die Verwendung von Schwesterchromatiden-Austauschen als mögliche Indikatoren für mutagene Wirkungen wird diskutiert.

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References

  • Bauchinger, M., Schmid, E., Schmidt, D.: Chromosomenanalyse bei Verkehrspolizisten mit erhöhter Bleilast. Mutat. Res. 16, 407–412 (1972)

    Google Scholar 

  • Beek, B., Obe, G.: Effect of lead acetate on human leukocyte chromosomes in vitro. Experientia (Basel) 30, 1006–1007 (1974)

    Google Scholar 

  • Brewen, J. G., Peacock, W. J.: The effect of tritiated thymidine on sister-chromatid exchange in a ring chromosome. Mutat. Res. 7, 433–440 (1969)

    Google Scholar 

  • Deknudt, G., Léonard, A., Ivanov, B.: Chromosome aberrations observed in male workers occupationally exposed to lead. Environm. Physiol. Biochem. 3, 132–138 (1973)

    Google Scholar 

  • Dudin, G., Beek, B., Obe, G.: The human leukocyte test system. I. DNA synthesis and mitoses in PHA-stimulated 2-day cultures. Mutat. Res. 23, 279–281 (1974)

    Google Scholar 

  • Dutrillaux, B., Fosse, A. M., Prieur, M., Lejeune, J.: Analyse des échanges de chromatides dans les cellules somatiques humaines. Traitement au BUDR (5 bromodéoxyuridine) et fluorescence bicolore par l'acridine orange. Chromosoma (Berl.) 48, 327–340 (1974)

    Google Scholar 

  • Forni, A., Secchi, G. C.: Chromosome changes in preclinical and clinical lead poisoning and correlation with biochemical findings. Proc. Intern. Symp. Environm. Health Asp. of Lead. Amsterdam, October 1972, 473–485 (1972)

  • Gatti, M., Olivieri, G.: The effect of X-rays on labelling pattern of M1 and M2 chromosomes in Chinese hamster cells. Mutat. Res. 17, 101–112 (1973)

    Google Scholar 

  • Gatti, M., Pimpinelli, S., Olivieri, G.: The frequency and distribution of isolabelling in Chinese hamster chromosomes after exposure to X-rays. Mutat. Res. 23, 229–238 (1974)

    Google Scholar 

  • Gibson, D. A., Prescott, D. M.: Induction of sister chromatid exchanges in chromosomes of rat kangaroo cells by tritium incorporated into DNA. Exp. Cell Res. 74, 397–402 (1972)

    Google Scholar 

  • Gibson, D. A., Prescott, D. M.: Sister chromatid exchanges in isolabelling. Exp. Cell Res. 83, 445–447 (1973)

    Google Scholar 

  • Ikushima, T., Wolff, S.: Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine-and 5-iododeoxyuridine substituted Chinese hamster chromosomes. Exp. Cell Res. 87, 15–19 (1974)

    Google Scholar 

  • Kato, H.: Induction of sister chromatid exchanges by UV light and its inhibition by caffeine. Exp. Cell Res. 82, 383–390 (1973)

    Google Scholar 

  • Kato, H.: Differential chromosomal sensitivities of aneusomic cell clones to UV light. Exp. Cell Res. 83, 55–62 (1974a)

    Google Scholar 

  • Kato, H.: Induction of sister chromatid exchanges by chemical mutagens and its possible relevance to DNA repair. Exp. Cell Res. 85, 239–247 (1974b)

    Google Scholar 

  • Kato, H.: Photoreactivation of sister chromatid exchanges induced by ultraviolet irradiation. Nature (Lond.) 249, 552–553 (1974c)

    Google Scholar 

  • Kato, H.: Spontaneous sister chromatid exchanges detected by a BUdR-labelling method. Nature (Lond.) 251, 70–72 (1974d)

    Google Scholar 

  • Kim, M. A.: Chromatidaustausch und Heterochromatinveränderungen menschlicher Chromosomen nach BUdR-Markierung. Nachweis mit Benzimidazolfluorochrom und Giemsafarbstoff. Humangenetik 25, 179–188 (1974)

    Google Scholar 

  • Korenberg, J. R., Freedlender, E. F.: Giemsa technique for the detection of sister chromatid exchanges. Chromosoma (Berl.) 48, 355–360 (1974)

    Google Scholar 

  • Latt, S. A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)

    Google Scholar 

  • Latt, S. A.: Localization of sister chromatid exchanges in human chromosomes. Science 185, 74–76 (1974a)

    Google Scholar 

  • Latt, S. A.: Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by mitomycin C. Proc. nat. Acad. Sci. (Wash.) 71, 3162–3166 (1974b)

    Google Scholar 

  • Léonard, A., Gerber, G. B.: Dangers génétiques d'une intoxication par le plomb. Ann. Gembloux 79, 109–119 (1973)

    Google Scholar 

  • Marin, G., Prescott, D. M.: The frequency of sister chromatid exchanges following exposure to varying doses of H3-thymidine or X-rays. J. Cell Biol. 21, 159–167 (1964)

    Google Scholar 

  • Obe, G., Sperling, K.: Zur Wirkung von Bleiacetat auf menschliche Leukocytenchromosomen in vitro. Arbeitsgruppe Blei der Kommission für Umweltgefahren des Bundesgesundheitsamtes Berlin, 1970

  • O'Riordan, M. L., Evans, H. J.: Absence of significant chromosome damage in males occupationally exposed to lead. Nature (Lond.) 247, 50–53 (1974)

    Google Scholar 

  • Peacock, W. J.: Chromosome duplication and structure as determined by autoradiography. Proc. nat. Acad. Sci. (Wash.) 49, 793–801 (1963)

    Google Scholar 

  • Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature (Lond.) 251, 156–158 (1974)

    Google Scholar 

  • Rommelaere, J., Susskind, M., Errera, M.: Chromosome and chromatid exchanges in Chinese hamster cells. Chromosoma (Berl.) 41, 243–257 (1973)

    Google Scholar 

  • Schmid, E., Bauchinger, M., Pietruck, S., Hall, G.: Die cytogenetische Wirkung von Blei in menschlichen peripheren Lymphocyten in vitro und in vivo. Mutat. Res. 16, 401–406 (1972)

    Google Scholar 

  • Schwanitz, G., Lehnert, G., Gebhart, E.: Chromosomenschäden bei beruflicher Bleibelastung. Dtsch. med. Wschr. 95, 1636–1641 (1970)

    Google Scholar 

  • Sperling, K., Weiss, G., Münzer, M., Obe, G.: Chromosomenuntersuchungen an bleiexponierten Arbeitern. Arbeitsgruppe Blei der Kommission für Umweltgefahren des Bundesgesundheitsamtes Berlin, 1970

  • Taylor, J. H.: The replication and organization of DNA in chromosomes. In: Molecular genetics (ed. J. H. Taylor), Vol. I, pp. 65–111. New York-London: Academic Press 1963

    Google Scholar 

  • Taylor, J. H., Woods, P. S., Hughes, W. L.: The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labelled thymidine. Proc. nat. Acad. Sci. (Wash.) 43, 122–128 (1957)

    Google Scholar 

  • Walen, K. H.: Spatial relationships in the replication of chromosomal DNA. Genetics 51, 915–929 (1965)

    Google Scholar 

  • Wolff, S., Bodycote, J., Painter, R. B.: Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation of different stages of the cell cycle: the necessity for cells to pass through S. Mutat. Res. 25, 73–81 (1974)

    Google Scholar 

  • Wolff, S., Perry, P.: Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma (Berl.) 48, 341–353 (1974)

    Google Scholar 

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Authors and Affiliations

  1. Institut für Genetik, Freie Universität Berlin, Berlin

    B. Beek & G. Obe

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  1. B. Beek
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  2. G. Obe
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Beek, B., Obe, G. The human leukocyte test system. Hum Genet 29, 127–134 (1975). https://doi.org/10.1007/BF00430349

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  • DOI: https://doi.org/10.1007/BF00430349

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