Abstract
Fine structural details of the parasitic yeastlike phase of Sporothrix schenckii contained in biopsy tissue from a naturally-occurring case of disseminated feline sporotrichosis are described and illustrated by electron microscopy. Both free and phagocytosed fungal cells were observed. The fungal cells were contained within an extracellular, electron transparent vacuolar area which was bounded by a limiting membrane of probable host origin. The yeastlike cells were characterized by a conspicuous layer of osmiophilic microfilaments which occurred along the outermost surface of the cell wall. In many yeastlike cells, scattered, membranebound vacuoles containing electron opaque material were observed in the cytoplasm. Asteroid bodies were not observed.
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Anderson, N.V., D. Ivoghli, W.E. Moore & H.W. Leipold. 1973. Cutaneous sporotrichosis in a cat: a case report. J.A.A.H.A. 9: 526–529.
Barbee, W. C., A. Ewert & E.M. Davidson. 1977. Sporotrichosis in the domestic cat. Amer. J. Pathol. 86: 281–284.
Belcher, R. W., J.F. Carney & F.G. Monahan. 1973. An electron microscopic study of phagocytosis of Candida albicans by polymorphonuclear leucocytes. Lab. Invest. 29: 620–627.
Carrada-Bravo, T. 1975. New observations on the epidemiology and pathogenesis of sporotrichosis. Ann. Trop. Med. Parasitol. 69: 267–273.
Collins, D.N. & M.R. Edwards. 1970. Filamentous forms of Blastomyces dermatitidis in mouse lung: Light and electron microscopy. Sabouraudia 7: 237–240.
Collins, D. N., I.A. Oppenheim & M.R. Edwards. 1971. Cryptococcus associated with systemic lupus erythematosus. Light and electron microscopic observations on a morphologic variant. Arch. Pathol. 91: 78–88.
Craig, E.L. & T. Suie. 1974. Histoplasma capsulatum in human ocular tissue. Arch. Ophthalmol. 91: 285–289.
de Brito, T., J.S. Furtado, R. Martius Castro & M. Manini. 1973. Intraepithelial parasitism as an infection mechanism in human paracoccidiodomycosis (South American blastomycosis). Virchows Arch. Abst. A Pathol. Anat. 361: 129–138.
Donelly, W.H. & E.J. Yunis. 1974. The ultrastructure of Coccidioides immitis. Study of a human infection. Arch. Pathol. 98: 227–232.
Dumont, A. & C. Piche. 1969. Electron microscopic study of human histoplasmosis. Arch. Pathol. 87: 168–178.
Dumont, A. & A. Robert. 1970. Electron microscopic study of phagocytosis of Histoplasma capsulatum by hamster peritoneal macrophages. Lab. Invest. 23: 278–286.
Edwards, M. R., M.A. Gordon, E.W. Lapa & W.C. Ghiorse. 1967. Micromorphology of Cryptococcus neoformans. J. Bacteriol. 94: 766–777.
Garrison, R. G., K.S. Boyd, & F. Mariat. 1975. Ultrastructural studies on the mycelial to yeast transformation of Sporothrix schenckii. J. Bacteriol. 124: 959–968.
Garrison, R. G., F. fnMariat, K.S. Boyd, & H. Fromentin. Ultrastructural observations of an unusual osmiophilic body in the hyphae of Sporothrix schenckii and Ceratocystis stenoceras. Ann. Microbiol. (Inst. Pasteur) 128B: 319–337.
Gutierrez, F., S.Y. Fu & H.I. Lurie. 1975. Cryptococcus histologically resembling histoplasmosis. A light and electron microscopic study. Arch. Pathol. 99: 347–352.
Jungerman, P.F. & R.M. Schwartzman. 1972. Veterinary medical mycology, pp. 31–39. Lea and Febiger, Philadelphia.
Kalina, M., Y. Kletter & M. Aronson. 1974. The interaction of phagocytes and the large-sized parasite Cryptococcus neoformans: Cytochemical and ultrastructural study. Cell Tiss. Res. 152: 165–174.
Keddie, F.M. 1966. Electron microscopy of Malassezia furfur in tinea versicolor. Sabouraudia 5: 134–137.
Kier, A. B., P. C. Mann & J. E. Wagner. 1979. Naturallyoccurring disseminated sporotrichosis in a cat: A case report. J. Amer. Vet. Med. Assoc. (In press).
Kirk, M. E., J. Lough & H.A. Warner. 1971. Histoplasma colitis: An electron microscopic study. Gastroenterol. 61: 46–54.
Lane, J. W., R.G. Garrison & M.F. Field. 1969. Ultrastructural studies on the yeastlike and mycelial phases of Sporotrichum schenckii. J. Bacteriol. 100: 1010–1019.
Lurie, H.I. 1971. Sporotrichosis in Human infection with fungi, actinomycetes and algae: Special edition of Hanbuch der speziellen pathologischen anatomie und histologie III/5, pp. 614–675, ed. by R.D. Baker. Springer-Verlag, Berlin.
Lurie, H.I. & W.J.S. Still. 1969. The ‘capsule’ of Sporotrichum schenckii and the evolution of the asteroid body. Sabouraudia 7: 64–70.
Mahvi, T.A., S.S. Spicer & N.J. Wright. 1974. Cytochemistry of acid mucosubstances and acid phosphatase in Cryptococcus neoformans. Canad. J. Microbiol. 20: 833–838.
Scott, D.W. 1977. Sporotrichosis. in Current Veterinary Therapy, Vol. VI. pp. 557–558, ed. by R.W. Kirk. W.B. Saunders, Philadelphia.
Scott, D. W., J. Bentinck-Smith & G.F. Haggerty. 1974. Sporotrichosis in three dogs. Cornell Vet. 64: 416–426.
Travassos, L. R., W. de Sousa, L. Mendonca-Previato & K.O. Lloyd. 1977. Location and biochemical nature of surface components reacting with concanavalin A in different cell types of Sporothrix schenckii. Exptl. Mycol. 1: 293–305.
Vanbreuseghem, R. 1973. Ultrastructure of Rhinosporidium seeberi. Int. J. Dermatol. 12: 20–28.
Werner, R.E., B.G. Levine, W. Kaplan, W. Hall, B.J. Nilles & M.D. O'Rourke. 1971. Sporotrichosis in a cat. J. Amer. Vet. Med. Assoc. 159: 407–412.
Williams, A.O. 1969. Pathology of phycomycosis due to Entomophthora and Basidiobolus species. Arch. Pathol. 87: 13–20.
Williams, A. O., F. Lichtenberg, J.H. Smith & F.D. Martinson. 1969. Ultrastructure of phycomycosis due to Entomophthora, Basidiobolus and associated ‘Splendore-Hoeppli’ phenomenon. Arch. Pathol. 87: 459–486.
Woodard, J.C. 1972. Electron microscopic study of lobomycosis (Loboa loboi). Lab. Invest. 27: 606–612.
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Garrison, R.G., Boyd, K.S., Kier, A.B. et al. Spontaneous feline sporotrichosis: A fine structural study. Mycopathologia 69, 57–62 (1979). https://doi.org/10.1007/BF00428603
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DOI: https://doi.org/10.1007/BF00428603