Abstract
The effect of white light on the malate oxidase of Sarcina lutea (Micrococcus luteus) membranes has been examined using a carotenoid-containing and a carotenoidless mutant. At least three photosensitive sites have been detected. Two of these are associated with the malate dehydrogenase complex (malate-menaquinone reductase) and are unaffected by membrane carotenoid. A third site which has been detected beyond the dehydrogenase complex, is protected by carotenoid since it can only be demonstrated in carotenoidless systems. A repair mechanism has been found for one of the two sites in the dehydrogenase complex.
Similar content being viewed by others
References
Anderson, S. M., Krinsky, N. I.: Protective action of carotenoid pigments against photodynamic action to liposomes. Photochem. Photobiol. 18, 403–408 (1973)
Barnes, E. M., Kaback, H. R.: Mechanisms of active transport in isolated membrane vesicles. I. The site of energy coupling between d-lactic dehydrogenase and β-galactoside transport in Escherichia coli membrane vesicles. J. biol. Chem. 246, 5518–5522 (1971)
Bishop, D. H. L., Pandya, K. P., King, H. K.: Ubiquinone and vitamin K in bacteria. Biochem. J. 83, 606–614 (1962)
Brodie, A. F., Gutnick, D. L.: Electron transport and oxidative phosphorylation in microbial systems. In: Electron and coupled energy transfer in biological systems, Vol. 1B (T. E. King, M. Klingenberg, eds.), pp. 599–681. New York: Dekker 1972
Brodie, A. F., Revsin, B., Kalra, V., Phillips, P., Bogin, E., Higashi, T., Murti, C. R. K., Cavari, B. Z., Marquez, E.: Biological function of terpenoid quinones. In: Natural substances formed biologically from mevalonic acid (T. W. Goodwin, ed.), pp. 119–143. London, Academic Press 1970
Epel, B. L.: Inhibition of growth and respiration by visible and near-visible light. In: Photophysiology, Vol. 8 (A. C. Giese, ed.), pp. 209–229. New York: Academic Press 1973
Erickson, S. K., Parker, G. L.: The electron transport system of Micrococcus luteus (Sarcina lutea). Biochim. biophys. Acta (Amst.) 180, 56–62 (1969)
Krinsky, N. I.: Carotenoid function. In: Carotenoids (O. Isler, ed.), pp. 669–716. Basel: Birkhäuser 1971
Kunisawa, R., Stanier, R. Y.: Studies on the role of carotenoid pigments in a chemoheterotrophic bacterium, Corynebacterium poinsettiae. Arch. Mikrobiol. 31, 146–156 (1958)
Mathews, M. M.: The effect of low temperature on the protection by carotenoids against lethal photosensitization in Sarcina lutea. Photochem. Photobiol. 3, 75–77 (1964)
Mathews, M. M., Sistrom, W. R.: Intracellular location of carotenoid pigments and some respiratory enzymes in Sarcina lutea. J. Bact. 78, 778–787 (1959)
Mathews, M. M., Sistrom, W. R.: Function of carotenoid pigments of Sarcina lutea. Arch. Mikrobiol. 35, 139–146 (1960)
Ninneman, H.: Photoinhibition of isolated complexes I, II and III of beef heart mitochondria. FEBS Letters 39, 353–358 (1974)
Prebble, J., Huda, A. S.: Sensitivity of the electron transport chain of pigmented and non-pigmented Sarcina membranes to photodynamic action. Photochem. Photobiol. 17, 255–264 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Prebble, J., Huda, S. The photosensitivity of the malate oxidase system of a pigmented strain and a carotenoidles mutant of Sarcina lutea (Micrococcus luteus) . Arch. Microbiol. 113, 39–42 (1977). https://doi.org/10.1007/BF00428577
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00428577