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Cytochemical detection of polysaccharides on the surface of the cell membrane complex in fungi

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Abstract

Cytochemical staining in toto (periodic acid, thiosemicarbazide, OSO4) revealed the presence of polysaccharide lamellae on the surface of the cell membrane complex of fungi. The membraneous clusters in the vacuolar bodies of Claviceps purpurea were covered with these lamellae at both surfaces, as it was also the case with the endoplasmic reticulum membranes, the tonoplast and the cytoplasmic membrane. In Saccharomyces cerevisiae, the polysaccharide lamellae were visible on the surface of the endoplasmic reticulum membranes and the plasmalemma; the strain revealed polysaccharide deposits also on the tonoplasts of small vacuoles and in glucanase vesicles. We assume that these observations give precision to the localization of the enzymes synthetizing the glycoprotein components of the fungal cell wall.

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Abbreviations

ER:

endoplasmic reticulum

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Authors and Affiliations

  1. Institute of Microbiology, Czechoslovak Academy of Sciences, Praha

    Josef Voříšek & Vladimir Pokorný

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  1. Josef Voříšek
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  2. Vladimir Pokorný
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Voříšek, J., Pokorný, V. Cytochemical detection of polysaccharides on the surface of the cell membrane complex in fungi. Arch. Microbiol. 102, 293–298 (1975). https://doi.org/10.1007/BF00428380

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  • DOI: https://doi.org/10.1007/BF00428380

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