Abstract
The haploid phase (myxamoebae-swarm cells) of the myxomycete Physarum flavicomum grew readily in chemically defined liquid media. The minimal medium contained salts, glucose, biotin, thiamine, hematin, glycine, l-arginine and l-methionine. Cell yields of 1.4x107 cells/ml were obtained in this medium in aerobic shake culture. These cells consumed about 35 μliters of oxygen/mg protein·hr in the minimal medium. The morphology of cells maintained in this medium appeared to be “normal”. l-valine replaced either glycine or l-methionine in the minimal medium but the growth rates and cell yields were reduced. Growth rates increased in media containing four, seven, or fourteen amino acids.
References
Goodman, E.M.: Axenic culture of the myxomycete Physarum polycephalum. J. Bact. 111, 242–247 (1972)
Gray, W.D., Alexopoulos, C.J.: Biology of the myxomycetes. New York: Ronald Press 1968
Henney, H.R., Jr., Asgari, M., Henney, M.R.: Growth of the haploid and diploid phases of Physarum flavicomum in the same partially defined media. Canad. J. Microbiol. 20, 967–970 (1974)
Henney, H.R., Jr., Henney, M.R.: Nutritional requirements for the growth in pure culture of the myxomycete Physarum rigidum and related species. J. gen. Microbiol. 53, 333–339 (1968a)
Henney, H.R., Jr.: Lynch, T.: Growth of Physarum flavicomum and Physarum rigidum in chemically defined minimal media. J. Bact. 99, 531–534 (1969)
Henney, M.R., Henney, H.R., Jr.: The mating-type systems of the myxomycetes Physarum rigidum and P. flavicomum. J. gen. Microbiol. 53, 321–332 (1968b)
Pigon, A., Morita, M.: Cell contact, cell-to-cell exchange, and conditioned medium in amoeba culture. Cytobiologie 8, 76–88 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Henney, H.R., Asgari, M. Growth of the haploid phase of the myxomycete Physarum flavicomum in defined minimal medium. Arch. Microbiol. 102, 175–178 (1975). https://doi.org/10.1007/BF00428365
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00428365