Abstract
A new bacteriochlorophyll has been isolated by thin layer chromatography from all strains of the brown-colored Chlorobiaceae Chlorobium phaeobacteroides and Chlorobium phaeovibrioides. The new bacteriochlorophyll e —like the bacteriochlorophylls c and d—represents the major amount of bacteriochlorophyll in the cells in addition to small amounts of bacteriochlorophyll a. Bacteriochlorophyll e can be differentiated from the bacteriochlorophylls c and d by its absorption maxima in aceton and its different R f -value in the thin layer chromatogram. The structure of the new bacteriochlorophyll e has been elucidated on the basis of mass spectra, 1H- and 13C-NMR-spectra, the UV/VIS-spectrum as well as IR-, ORD-, and CD-spectra. The new bacteriochlorophyll has the same relationship to bacteriochlorophyll c as chlorophyll b from green plants to chlorophyll a; therefore, bacteriochlorophyll e represents the first formyl-substituted chlorophyll from bacteria. Similar to the bacteriochlorophylls c and d, the new bacteriochlorophyll e consists of a mixture of at least three homologues which differ from each other by different substituents on the pyrrol rings II and III.
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Abbreviations
- DSM:
-
Deutsche Sammlung von Mikroorganismen, Göttingen
- Bchl.:
-
bacteriochlorophyll
- Bph.:
-
bacteriopheophytin
- P:
-
phytol
- Gg:
-
geranylgeraniol
- F:
-
farnesol
- C:
-
Chlorobium
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This work was made possible by the technology program of the Bundesministerium für Forschung und Technologie.
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Gloe, A., Pfennig, N., Brockmann, H. et al. A new bacteriochlorophyll from brown-colored chlorobiaceae. Arch. Microbiol. 102, 103–109 (1975). https://doi.org/10.1007/BF00428353
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DOI: https://doi.org/10.1007/BF00428353