Abstract
The regulation of the synthesis of alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase was investigated in the methanol-utilizing yeast Hansenula polymorpha. The organism was found to synthesize immunologically identical alcohol oxidases during growth on glycerol and methanol. Growth on glycerol, however, was not dependent on the alcohol oxidase, as was shown with a mutant without alcohol oxidase protein. Similarly it was shown with a catalase activity negative mutant that high catalase activity during growth on glycerol was not a prerequisite for the utilization of this substrate, though absolutely required for growth on methanol.
Experiments were conducted with mixed substrates to study the influence of methanol on alcohol oxidase synthesis. In batch cultures, growth on ribose plus methanol resulted in an enhanced rate of alcohol oxidase synthesis as compared to ribose alone. In continuous cultures, (D=0.1 h-1) addition of methanol to glycerol-, glucose-, or sorbose-limited cultures gave rise to increased alcohol oxidase activity of up to 20 U/mg, which is about by 2 times higher than the specific activity used for growth on methanol alone. The increase in specific activity of the dissimilatory enzymes on the mixed substrates is partly due to methanol per se, as was shown by a mutant unable to dissimilate or assimilate methanol.
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References
Chiriacy, M.: A yeast mutant with glucose-resistant formation of mitochondrial enzymes. Molec. Gen. Genet. 129, 329–335 (1978)
Dijken, J. P. v., Otto, R., Harder, W.: Growth of Hansenula polymorpha in a methanol-limited chemostat. Physiological responses due to the involvement of methanol oxidase as a key enzyme in methanol metabolism. Arch. Microbiol. 111, 137–144 (1976)
Dijken, J. P. v.: Metabolic compartmentation in methanol-utilizing yeasts. In: Proceedings of the VIth International Specialized Symposium on Yeasts. (Chair of Genetics and Microbiology, ed.) ENSAM-CRAM (1978)
Dijken, J. P. v., Quayle, J. R.: Fructose metabolism in four Pseudomonas species. Arch. Microbiol. 114, 281–286 (1977)
Dijken, J. P. v., Harder, W., Beardsmore, A. J., Quayle, J. R.: Dihydroxyacetone: An intermediate in the metabolism of methanol by yeasts? FEMS Lett. 4, 97–102 (1978)
Dix, D.: Untersuchungen zur Assimilation des Methanols in der Hefe Candida boidinii. Diss., Univ. Braunschweig 1979
Eggeling, L., Sahm, H., Wagner, F.: Induction of FMN adenylyl-transferase in the methanol-utilizing yeast Candida boidinii. FEMS Lett. 1, 205–210 (1977)
Eggeling, L., Sahm, H.: Derepression and partial insensitivity to carbon catabolite repression of the methanol dissimilating enzymes in Hansenula polymorpha. Eur. J. Appl. Microbiol. Biotechnol. 5, 197–202 (1978)
Egli, T., v. Dijken, J. P., Veenhuis, M., Harder, W., Fiechter, A.: Methanol metabolism in yeasts: Regulation of the synthesis of catabolic enzymes. Arch. Microbiol. 124, 115–121 (1980)
Entian, K. D., Zimmermann, F. K., Scheel, J.: A partial defect in carbon catabolite repression in mutants of Saccharomyces cerevisiae with reduced hexose phosphorylation. Molec. Gen. Genet. 156, 99–105 (1977)
Gornall, A. G., Bardawill, C. J., David, M. M.: Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 177, 751–766 (1949)
Jaspers, H. T. A., Christiansen, K., Stevenick, J.: An improved method for the preparation of yeast enzymes in situ. Biochem. Biophys. Res. Comm. 65, 1434–1439 (1975)
Kato, N., Omori, Y., Tani, Y., Ogata, K.: Alcohol oxidases of Kloeckera sp. and Hansenula polymorpha. Catalytic properties and subunit structures. Eur. J. Biochem. 64, 341–350 (1976)
Magasanik, B.: Catabolite repression. In: Cold Spring Harbour symposium on quantitative biology, Vol. XXVI (L. Frisch, ed.), pp. 249–256. New York: The Long Island Biological Association 1961
O'Connor, M., Quayle, J. R.: Mutants of Hansenula polymorpha and Candida boidinii impaired in their ability to grow on methanol. J. Gen. Microbiol. 113, 203–208 (1979)
Roggenkamp, R., Sahm, H., Wagner, F.: Microbial assimilation of methanol. Induction and function of catalase in Candida boidinii. FEBS Lett. 41, 283–286 (1974)
Sahm, H.: Metabolism of methanol by yeasts. Adv. Biochem. Eng. 6, 77–103 (1977)
Sahm, H., Wagner, F.: Microbial assimilation of methanol. The ethanol- and methanol-oxidizing enzymes of the yeast Candida boidinii. Eur. J. Biochem. 36, 250–256 (1973)
Schütte, H., Flossdorf, J., Sahm, H., Kula, M.-R.: Furification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii. Eur. J. Biochem. 62, 151–160 (1976)
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Eggeling, L., Sahm, H. Regulation of alcohol oxidase synthesis in Hansenula polymorpha: Oversynthesis during growth on mixed substrates and induction by methanol. Arch. Microbiol. 127, 119–124 (1980). https://doi.org/10.1007/BF00428015
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DOI: https://doi.org/10.1007/BF00428015