Abstract
Human platelets possess high-affinity 3H-imipramine binding sites. A study in ten healthy volunteers showed that platelet preparations produced by mechanical disruption contained varying proportions of intact platelets, as measured by the cytoplasmic marker enzyme LDH. This may invalidate the protein reference. Higher proportions of membranes in the preparations lead to higher B max values of imipramine binding (fmol/mg protein). The use of intact platelets in imipramine binding studies is therefore preferable to membrane preparations, particularly in studies where the interindividual variation of binding parameters is of interest.
This is a preview of subscription content, log in via an institution to check access.
References
Asarch KB, Shih JC, Kulcsàr A (1980) Decreased 3H-imipramine binding in depressed males and females. Comm Psychopharmacol 4:425–432
Barber AJ, Jamieson GA (1970) Isolation and characterization of plasma membranes from human platelets. J Biol Chem 245:6357–6365
Barber AJ, Pepper DS, Jamieson GA (1971) A comparison of methods for platelet lysis and the isolation of platelet membranes. Thromb Haemostas 26:38–57
Briley MS, Langer SZ, Raisman R (1980) Tritiated imipramine binding sites are decreased in platelets of untreated depressed patients. Science 209:303–305
Briley MS, Raisman R, Langer SZ (1979) Human platelets possess highaffinity binding sites for 3H-imipramine. Eur J Pharmacol 58:347–348
Da Prada M, Richards JG, Kettler R (1981) Amine storage organelles in platelets. In: Gordon JL (ed) Platelets in biology and pathology — 2. Elsevier/North-Holland Biomedical Press, Amsterdam New York Oxford, pp 107–145
Langer SZ, Briley MS, Raisman R, Henry JF, Morselli PL (1980) Specific 3H-imipramine binding in human platelets, Influence of age and sex. Naunyn-Schmiedeberg's Arch Pharmacol 313:189–194
Paul SM, Rehavi M, Skolnick P, Ballenger JC, Goodwin FK (1981) Depressed patients have decreased binding of tritiated imipramine to platelet serotonin ‘transporter’. Arch Gen Psychiatry 38:1315–1317
Paul SM, Rehavi M, Skolnick P, Goodwin FK (1980) Demonstration of specific “high-affinity” binding sites for 3H-imipramine on human platelets. Life Sci 26:953–959
Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83: 346–356
Raisman R, Briley MS, Langer SZ (1979) Specific tricyclic antidepressant binding sites in rat brain. Nature 281:148–150
Rehavi M, Paul SM, Skolnick P, Goodwin FK (1980) Demonstration of specific high-affinity binding sites for 3H-imipramine in human brain. Life Sci 26:2273–2279
Talvenheimo J, Nelson PJ, Rudnick G (1979) Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport. J Biol Chem 254:4631–4635
Wennogle LP, Beer B, Meyerson LR (1981) Human platelet imipramine recognition sites: Biochemical and pharmacological characterization. Pharmacol Biochem Behav 15:975–982
Author information
Authors and Affiliations
Additional information
Supported by Deutsche Forschungsgemeinschaft
Rights and permissions
About this article
Cite this article
Friedl, W., Propping, P. & Weck, B. 3H-Imipramine binding in platelets: Influence of varying proportions of intact platelets in membrane preparations on binding. Psychopharmacology 80, 96–99 (1983). https://doi.org/10.1007/BF00427505
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00427505