Abstract
A close genetic relationship among strains of Oerskovia turbata, O. xanthineolytica and various coryneforms is indicated by DNA-DNA reassociation studies. O. xanthineolytica shares high homology values (over 60%) with Cellulomonas cartae, Nocardia cellulans, Brevibacterium fermentans and Corynebacterium manihot. O. turbata and other cellulomonads show lower DNA homology values (20–25%) which are still high enough, however, to indicate a relationship at the genus level. Based on these data and supported by comparative analysis of the ribosomal 16 S RNA and the similarity of peptidoglycan types, the transfer of Oerskovia species into the genus Cellulomonas is justified.
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Bergey DH, Breed RS, Hammer RW, Harrison FC, Muntoon FM (1923) Bergey's Manual of Determinative Bacteriology, 1st ed. The Williams and Wilkins Co, Baltimore
Bousfield IJ (1972) A taxonomic study of some coryneform bacteria. J Gen Microbiol 71:441–455
Burton K (1968) Determination of DNA concentration with diphenylamine. In: Methods in enzymology, Vol XII, B 163–166
Bradley SG (1973) Relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid reassociation. J Bacteriol 113:645–651
Collins MD, Goodfellow M, Minnikin DE (1979) Isoprenoid quinones in the classification of coryneform and related bacteria. J Gen Microbiol 110:127–136
Crombach WHJ (1978) DNA base ratios and hybridisation studies of coryneform bacteria, Mycobacteria and Nocardiae. In: Callely AG, Bousfield IJ (eds) Coryneform bacteria. Academic Press, p 161–179
Cummins CS (1962) Chemical composition and antigenic structure of cell walls of Corynebacterium, Mycobacterium, Nocardia, Actinomyces and Arthrobacter. J Gen Microbiol 28:35–50
Darby KG, Jones AS, Kennedy JF, Walker RT (1970) Isolation and analysis of the nucleic acids and polysaccharides from Clostridium welchii. J Bact 103:159–165
Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646
Farina G, Bradley SG (1970) Reassociation of deoxyribonucleic acids from Actinoplanes and other Actinomycetes. J Bacteriol 102:30–35
Fiedler F, Schleifer KH, Cziharz B, Interschick E, Kandler O (1970) Murein types in Arthrobacter, Brevibacteria, Corynebacteria and Microbacteria. Publ Fac Sci Univ J E Purkyne Brno 47:111–122
Fiedler F, Kandler O (1973) Die Mureintypen der Gattung Cellulomonas. Bergey et al. Arch Mikrobiol 89:41–50
Frank-Kamenetskii MD (1971) Simplification of the empirical relationship between melting temperature of DNA, its GC content and concentration of sodium ions in solution. Biopolymers 10:2673
Gillespie D, Spiegelman S (1965) A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol 12:829–842
Goodfellow M (1971) Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69:33–80
Goodfellow M, Minnikin DE (1977) Nocardioform bacteria. Ann Rev Microbiol 31:159–180
Johnson JL (1973) Use of nucleic acid homologies in the taxonomy of anaerobic bacteria. Int J Syst Bacteriol 23:359–375
Jones D (1975) A numerical taxonomic study of coryneform and related bacteria. J Gen Microbiol 87:52–96
Jones LA, Bradley SG (1964) Phenetic classification of actinomycetes. Dev Ind Microbiol 5:267–272
Keddie RM, Cure L (1977) The cell wall composition and distribution of free mycolic acid in named strains of coryneform bacteria and in isolates from various natural sources. J Appl Bacteriol 42:229–252
Lechevalier MP, Lechevalier H (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443
Lechevalier MP, Lechevalier H, Horan AC (1973) Chemical characteristics and classification of nocardiae. Can J Microbiol 19:965–972
marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218
Marmur J, Doty P (1961) Thermal renaturation of deoxyribonucleic acids. J Mol Biol 3:208–218
McConaughy BL, Laird CD, McCarthy BJ (1969) Nucleic acid reassociation in formamide. Biochemistry 8:3289–3295
Meyer SA, Schleifer KH (1978) Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Arch Microbiol 117:183–188
Minnikin DE, Goodfellow M, Collins MD (1978a) Lipid composition in the classification of coryneform and related taxa. In: Bousfield IJ, Callely AG (eds) Coryneform bacteria. Academic Press, New York, pp 85–160
Minnikin DE, Goodfellow M, Alshamaony L (1978b) Mycolic acids in the classification of nocardioform bacteria. In: Modarski M, Kurylowicz W, Jeljaszwicz J (eds) Proceedings of the international symposium on Nocardia and Streptomyces. Fischer, Stuttgart, pp 63–66
Minnikin DE, Collins MD, Goodfellow M (1978c) Menachinone patterns in the classification of nocardioform and related bacteria. In: Modarski M, Kurylowicz W, Jeljaszwicz J (eds) Proceedings of the International Symposium on Nocardia and Streptomyces. Fischer, Stuttgart, pp 85–90
Prauser H, Lechevalier MP, Lechevalier H (1970) Description of Oerskovia gen. n. to harbor Øerskov's motile Nocardia. Appl. Microbiol 19:534
Rogosa M, Cummins CS, Lelliot RA, Keddie RM (1974) Actinomycetes and related organisms. In: Buchanan RE, Gibbons NE (eds) Bergey's Manual of Determinative Bacteriology, 8th ed. The Williams and Wilkins Co., Baltimore, pp 559–861
Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
Schleifer KH, Kandler O (1967) Zur chemischen Zusammensetzung der Zellwände der Streptokokken. I. Zur Aminosäuresequenz des Mureins von Str. thermophilus und Str. faecalis. Arch Microbiol 57:335–364
Seidl PH, Faller AH, Loider R, Schleifer KH (1980) Peptidoglycan types and cytochrome patterns of strains of Oerskovia turbata and O. xanthineolytica. Arch Microbiol 127:173–178
Stackebrandt E, Fiedler F (1979) DNA-DNA homology studies among strains of Arthrobacter and Brevibacterium. Arch Microbiol 120:289–295
Stackebrandt E, Fiedler F, Kandler O (1978) Peptidoglycan type and cell wall polysaccharide composition of Cellulomonas cartalyticum and some coryneform organisms. Arch Microbiol 117:115–118
Stackebrandt E, Kandler O (1979) Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid homology, and proposal of seven neotype strains. Int J Syst Bacteriol 29:273–282
Stackebrandt E, Woese CR (1979) A phylogenetic dissection of the family Micrococcaceae. Curr Microbiol 2:317–322
Stackebrandt E, Lewis BJ, Woese CR (1980) The phylogenetic structure of the coryneform group of bacteria. Zbl Bakt I Abt Orig C 1 137–149
Steigerwalt AG, Fanning GR, Fife-Asbury MA, Brenner DJ (1975) DNA relatedness among species of Enterobacter and Serratia. Can. J. Microbiol 22:441–455
Sukapure RS, Lechevalier MP, Reber H, Higgins MC, Lechevalier H, Prauser H (1970) Motile nocardoid Actinomycetales. Appl Microbiol 19:527–533
Tewfik EM, Bradley SG (1967) Characterisation of deoxyribonucleic acids from streptomycetes and nocardiae. J Bacteriol 94:1994–2000
Van Niel CB (1946) The classification and natural relationships of bacteria. Cold Spring Harbor Symp Quant Biol 11:285–301
Woese SR, Fox GE (1977) Phylogenetic structure of the prokaryotic domain. The primary kingdoms. Proc Natl Acad Sci USA (Wash) 74:5088–5090
Yamada Y, Inouye G, Tahara Y, Kondo K (1976) The menaquinone system in the classification of coryneform and nocardioform bacteria and related organisms. J Gen Appl Microbiol 22:203–214
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This paper is respectively dedicated to our teacher and mentor, Professor Dr. O. Kandler, on the occasion of his 60th birthday.
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Stackebrandt, E., Häringer, M. & Schleifer, KH. Molecular genetic evidence for the transfer of Oerskovia species into the genus Cellulomonas . Arch. Microbiol. 127, 179–185 (1980). https://doi.org/10.1007/BF00427191
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DOI: https://doi.org/10.1007/BF00427191