Summary
The distribution of several markers of keratinocyte differentiation was studied in normal epidermis, basal cell carcinomas (BCCs), and squamous cell carcinomas (SCCs) using the immunoperoxidase technique on frozen sections of punch biopsy specimens. As markers a panel of chain-specific monoclonal antibodies (MoAbs) directed against cytokeratin (CK) 4, 8, 10, 13, 18, and 19, a polyclonal antiserum against involucrin, as well as a MoAb against the epidermal growth factor (EGF) receptor were used. In 15 out of 19 BCCs tested, expression of CK 8 was seen. Only a few individual cells in a limited number of BCCs showed positive staining for CK 4, 18, or 19. No expression of CK 10 was seen except for some foci of cell keratinization. Involucrin was not found in BCCs except for some squamous horn cysts. In all BCC cells expression of EGF receptor was found. In the suprabasal layers of normal epidermis from SCC patients, positive staining for CK 10 was seen. A few individual cells in a limited number of SCCs showed positive staining for CK 4, 8, or 18. Involucrin was expressed in the center of SCCs and in the upper layers of normal epidermis. Expression of EGF receptor was found in all SCC cells. These results demonstrate differences in cellular origin and differentiation between BCC and SCC.
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References
Boonstra J, de Laat SW, Ponec M (1985) Epidermal growth factor receptor expression related to differentiation, capacity in normal and transformed keratinocytes. Exp Cell Res 161:421–433
Carter DM (1987) Basal cell carcinoma. In: Fitzpatrick TB (ed) Dermatology in general medicine. McGraw-Hill, New York, pp 259–265
Dale BA, Holbrook KA, Kimball JR, Hoff M, Sun TT (1985) Expression of epidermal keratins and filaggrin during human fetal skin development. J Cell Biol 101:1257–1269
Debus E, Moll R, Franke WW, Weber K, Osborn M (1984) Immunohistochemical distinction of human carcinomas by cytokeratin typing with monoclonal antibodies. Am J Pathol 114:121–130
Defize IHK, Moolenaar WH, van der Saag PT, de Laat SW (1986) Dissociation of cellular responses to epidermal growth factor using anti-receptor monoclonal antibodies. EMBO 5:1187–1192
Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042
Golitz LE, Norris DA, Luekens CA, Charles DM (1980) Nevoid basal cell, carcinoma syndrome: multiple basal cell carcinomas of the palms after radiation therapy. Arch Dermatol 116:1159–1163
Green MR, Phil D, Couchman JR (1985) Differences in human skin between the epidermal growth factor receptor distribution detected by EGF binding and monoclonal antibody recognition. J Invest Dermatol 85:239–245
Gusterson B, Lowley G, Smith JA, Ozanne B (1984) Cellular localisation of human epidermal growth factor receptor. Cell Biol Int Rep 8:649–657
Huszar M, Gigi-Leitner O, Moll R, Franke WW, Seiger B (1986) Selective markers for stratification and squamous cell carcinomas. Differentiation 31:141–153
Lane EB (1982) Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization. J Cell Biol 92:665–673
Lane EB, Bartek J, Purknis PE, Leigh IM (1985) Keratin antigens in differentiating skin. Ann NY Acad Sci 455: 241–258
Lever WF, Schaumburg-Lever G (1975) Histopathology of the skin. Lippincott, Philadelphia, pp 499–503
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11–24
Moll R, Ranke WW, Volc-Platzer B, Krepler R (1982) Different keratin polypeptides in epidermis and other epithelia of human skin: a specific cytokeratin of molecular weight 46,000 in epithelia, of the pilosebaceous tract and basal cell epitheliomas. J Cell Biol 95:285–295
Moll R, Moll I, Franke WW (1984) Differences of expression of cytokeratin polypeptides in various epithelial skin tumors. Arch Dermatol Res 276:349–363
Nakane PK, Pierce GB (1966) Enzyme labeled, antibodies: preparation and application for the localization of antigens. J Histochem Cytochem 14:929–931
Nanney LB, McKanna JA, Stoscheck CM, Carpenter G, King LE (1984) Visualisation of epidermal growth factor receptors in human epidermis. J Invest Dermatol 82:165–169
Ozanne B, Richards CS (1986) Over-expression of EGF receptor is a hallmark of squamous cell carcinomas. J Pathol 149:9–14
Ramaekers, FCS, Puts, JJG, Moesker O, Kant A, Huysmans A, Haag D, Jap PHK, Herman CJ, Vooijs CP (1983) Antibodies to intermediate filament proteins in the immuno-histochemical identification of human tumors: an overview. Histochem J 15:691–713
Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooys P (1987) Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170:235–249
Rice RH, Green H (1979) Fresence in human epidermis of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions. Cell 18: 681–694
Rice RH, Pinkus GS, Warhol MJ, Antonioli DA (1984) Involucrin: biochemistry and immunohistochemistry In: de Lellis RA (ed) Advances in immunohistochemistry. Masson, New York, pp 111–125
Said JW, Sassoon AF, Shintaku IP, Banks-Schlegel S (1984) Involucrin in squamous and basal cell carcinomas of the skin: an immunohistochemical study. J Invest Dermatol 82:449–452
Smoller BR, Kwan TH, Said JW, Banks-Schlegel SP (1986) Keratoacanthoma and squamous cell carcinoma of the skin: immunohistochemical localization of involucrin and keratin proteins. J Am Acad Dermatol 14:226–234
Stoscheck CM, King, LE (1986) Role of epidermal growth factor in carcinogenesis. Cancer Res 46:1030–1037
Sun TT, Eichner R, Schermer A, Cooper D, Nelson WG, Weiss RA (1984) Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: a unifying model. Cancer Cells 1:169–176
Thomas P, Said JW, Nash G, Banks-Schlegel, S (1984) Profiles of keratin protein in basal and squamous cell carcinomas of the skin. Lab Invest 1:36–41
van Muijen GNP, Ruiter DJ, Franke WW, Achtstätter T, Haasnoot WHB, Ponec M, Warnaar SO (1986) Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res 162:97–113
van Muijen GNP, Ruiter DJ, Warnaar SO (1987) Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest 57:359–369
van Muijen GNP, Warnaar SO, Ponec M (1987), Differentiation related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn and adult epidermis. Exp Cell Res 171:331–345
Viac J, Reano A, Thivolet J (1982) Cytokeratins in human basal and squamous cell carcinomas: biochemical, immuno-histological findings and comparisons with normal epithelia. J Cutan Pathol 9:377–390
Watt FM, Green H (1982) Stratification and terminal differentiation of cultured epidermal cells. Nature 295:434–436
Weiss RA, Guillet GYA, Freedberg IM, Farmer ER, Small EA, Weiss MM, Sun TT (1983) The use of monoclonal antibody to keratin in human epidermal disease: alternations in immunohistochemical staining pattern. J Invest Dermatol 81:224–230
Yamamoto T, Kamata N, Kawano H, Shimizu S, Kuroki T, Toyoshima K, Rikimaru K, Nomura N, Ishizaki R, Pastan I, Gamou S, Shimizu N (1986) High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines. Cancer Res 46:414–416
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Lavrijsen, A.P.M., Tieben, L.M., Ponec, M. et al. Expression of EGF receptor, involucrin, and cytokeratins in basal cell carcinomas and squamous cell, carcinomas of the skin. Arch Dermatol Res 281, 83–88 (1989). https://doi.org/10.1007/BF00426583
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DOI: https://doi.org/10.1007/BF00426583