Summary
In addition to the cryptic lambdoid prophage genes that are known to reside at the rac locus in Escherichia coli K12 strains, a second cryptic lambdoid prophage has been located near the gal operon. This prophage was shown to contain DNA that is homologous to the QSR genes of λ phage.
This is a preview of subscription content, log in via an institution to check access.
References
Anilionis A, Riley M (1980) Conservation and variation of nucleotide sequences within related bacterial genomes: Escherichia coli strains. J Bacteriol 143:355–365
Bachman BJ, Low KB (1980) Linkage map of Escherichia coli K-12, Edition 6. Microbiol Rev 44:1–56
Clewell DF, Helinski DR (1969) Supercoiled circular DNA-protein complex in Escherichia coli: Purification and induced conversion to open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166
Diaz R, Barnsley P, Pritchard RH (1979) Location and characterization of a new replication origin in the E. coli K12 chromosome. Mol Gen Genet 175:151–157
Diaz R, Pritchard RH (1978) Cloning of replication origins from the E. coli K12 chromsome. Nature (London) 275:561–564
Evans R, Seeley NR, Kuempel PL (1979) Loss of rac locus DNA in merozygotes of Escherichia coli K12. Mol Gen Genet 175:245–250
Henderson D, Weil J (1975) The nature and origin of a class of essential gene substitutions in bacteriophage λ. Virology 67:124–135
Herskowitz I, Signer E (1970) Control of transcription from the strand of bacteriophage lambda. Cold Spring Harb Symp Quant Biol 35:355–368
Herskowitz I, Signer E (1974) Substitution mutations in bacteriophage λ with new specificity for late gene expression. Virology 61:112–119
Jacob F, Wollman EL (1954) Etude genetique d'un bacteriophage tempere d'Escherichia coli. Ann Inst Pasteur (Lille) 87:653–673
Kaiser K, Murray NE (1979) Physical characterization of the “Rac-prophage” in E. coli K12. Mol Gen Genet 175:159–174
Low KB (1973) Restoration by the rac locus of recombinant forming ability in recB- and recC- merozygotes of E. coli K12. Mol Gen Genet 122:119–130
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Sato K, Campbell A (1970) Specialised transduction of galactose by lambda phage from a deletion lysogen. Virology 41:474–487
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–518
Strathern A, Herskowitz I (1975) Defective prophage in E. coli K12 strains. Virology 41:474–487
Author information
Authors and Affiliations
Additional information
Communicated by F. Stahl
Rights and permissions
About this article
Cite this article
Anilionis, A., Ostapchuk, P. & Riley, M. Identification of a second cryptic lambdoid prophage locus in the E. coli K12 chromosome. Molec. Gen. Genet. 180, 479–481 (1980). https://doi.org/10.1007/BF00425865
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425865