Abstract
The metabolism of trehalose in wild type cells of Escherichia coli and Salmonella typhimurium has been investigated. Intact cells of Escherichia coli (grown on trehalose) accumulated [14C]-trehalose as [14C]-trehalose 6-phosphate. Toluene-treated cells catalyzed the synthesis of the [14C]-sugar phosphate from [14C]-trehalose and phosphoenolpyruvate; ATP did not serve as phosphoryl donor. Trehalose 6-phosphate could subsequently be hydrolyzed by trehalose 6-phosphate hydrolase, an enzyme which catalyzes the hydrolysis of the disaccharide phosphate into glucose and glucose 6-phosphate. Both Escherichia coli and Salmonella typhimurium induced this enzyme when they grew on trehalose.
These findings suggest that trehalose is transported in these bacteria by an inducible phosphoenolpyruvate:trehalose phosphotransferase system.
The presence of a constitutive trehalase was also detected.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanosulfonic acid
- PEP:
-
phosphoenolpyruvate
- PTS:
-
phosphoenolpyruvate: glycose phosphotransferase system
- O.D.:
-
optical density
References
Becerra de Lares L, Ratouchniak J, Casse F (1977) Chromosomal location of gene governing the trehalose utilization in Escherichia coli K-12. Molec Gen Genet 152:105–108
Bhumiratana A, Anderson RL, Costilow RN (1974) Trehalose metabolism by Bacillus popilliae. J Bacteriol 119:484–493
Hengstenberg W, Egan JB, Morse ML (1968) Carbohydrate transport in Staphylococcus aureus. The nature of the derivatives accumulated. J Biol Chem 243:1881–1885
Lowry OH, Rosebrough HJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Boca Raton, pp 431–435
Nelson N (1944) A photometric determination of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380
Postma PW, Roseman S (1976) The bacterial phosphoenolpyruvate: sugar phosphotransferase system. Biochim Biophys Acta 457:213–257
Saint Pierre ML (1968) Isolation and mapping of Salmonella typhimurium mutants defective in the utilization of trehalose. J Bacteriol 95:1185–1186
Simoni R, Hays J, Nakazawa T, Roseman S (1973) Sugar transport. VI. Phosphoryl transfer in the lactose phosphotransferase system of Staphylococcus aureus. J Biol Chem 248:957–965
Somogyi M (1945) A new reagent for the determination of sugars. J Biol Chem 160:61–68
Thompson J, Chassy BM (1981) Uptake and metabolism of sucrose by Streptococcus lactis. J Bacteriol 147:543–551
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maréchal, L.R. Transport and metabolism of trehalose in Escherichia coli and Salmonella typhimurium . Arch. Microbiol. 137, 70–73 (1984). https://doi.org/10.1007/BF00425810
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00425810