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Mutants of Phycomyces with abnormal phototropism induced by ICR-170

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Summary

Twenty two mutants with abnormal phototrophism (genotype mad) were obtained from the wild type by mutagenesis with ICR-170. These mutants were tested for their sensory responses and grouped into two classes, based on the phenotypic classification scheme of Bergman et al. (1973). There were twenty class 1 mutants and two class 2 mutants. Complementation tests revealed that all class 2 mutants carry a mutation in the madD gene and all class 1 mutants tested carry a mutation in the madC gene. Recombination analysis between several madC alleles showed that all the madC alleles tested were in the same locus. No new gene affecting the light sensory pathway was found and none of these mutants were totally blind.

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Biología, Universidad de Salamanca, Salamanca, Spain

    M. I. Alvarez & A. P. Eslava

  2. Biology Laboratory, Education Faculty, Yamagata University, Yamagata, Japan

    T. Ootaki

Authors
  1. M. I. Alvarez
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  2. T. Ootaki
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  3. A. P. Eslava
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Communicated by G. Melchers

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Alvarez, M.I., Ootaki, T. & Eslava, A.P. Mutants of Phycomyces with abnormal phototropism induced by ICR-170. Molec. Gen. Genet. 191, 507–511 (1983). https://doi.org/10.1007/BF00425770

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  • DOI: https://doi.org/10.1007/BF00425770

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