Summary
We have identified p33 as a cell wall protein in carrot storage roots that increases in the cell wall after wounding, using antibodies raised against a synthetic peptide derived from the p33 cDNA sequence. The mRNA encoding p33 increases markedly within 1 h after wounding in carrots, indicating that p33 may be involved in one of the early cellular responses to physical damage. In contrast with extensin, p33 mRNA does not accumulate in carrot roots in response to ethylene or in carrot suspension culture cells incubated in the presence of a crude endogenous elicitor fraction. These results indicate that the wound-induced signals responsible for the synthesis of p33 and extensin after wounding are different. Protein immunologically related to carrot p33 and RNA transcripts homologous to p33 cDNA sequences were detected in the apical hook of the soybean seedling indicating that a cell wall protein related to p33 is also synthesized during plant development.
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Communicated by R.B. Goldberg
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Tierney, M.L., Wiechert, J. & Pluymers, D. Analysis of the expression of extensin and p33-related cell wall proteins in carrot and soybean. Molec. Gen. Genet. 211, 393–399 (1988). https://doi.org/10.1007/BF00425691
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DOI: https://doi.org/10.1007/BF00425691