Abstract
The elevated soluble cytochrome P-450 content of Streptomyces griseolus cells found after growth in the presence of sulfonylurea herbicides has been shown to be the result of the appearance of one predominant cytochrome P-450 form. This cytochrome P-450 is the major soluble protein found to increase in amount following herbicide treatment, and functions as part of a sulfometuron methyl hydroxylase system. A second minor inducible cytochrome P-450 has been observed only in cells grown in the presence of chlorimuron ethyl, and a third cytochrome P-450 has been found to be present in all cells independent of the presence of sulfonylurea inducers. The three cytochrome P-450 isozymes are distinguishable primarily by their anion exchange properties; however, spectral properties, substrate inducibility, and enzymatic activity provide several further distinguishing features. The recognition of these inducible, xenobiotic metabolizing cytochromes P-450 in S. griseolus provides the only known description of monooxygenase proteins related to herbicide metabolism in bacteria.
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Abbreviations
- Chlorsulfuron:
-
2-chloro-N-[4-methoxy-6-methyl-1,3,5-triazin-2-yl) aminocarbonyl]benzenesulfonamide
- sulfometuron methyl:
-
N-[(4,6-dimethylpyrimidine-2-yl)aminocarbonyl]-2-methoxycarbonyl-benzenesulfonamide
- chlorimuron ethyl:
-
N-[(4-chloro-6-methoxypyrimidine-2-yl)aminocarbonyl]2-ethoxycarbonylbenzene-sulfonamide
- LDS:
-
lithium dodecyl sulfate
References
Corcoran JW, Vygantas AM (1982) Accumulation of 6-deoxyerythronolide B in a normal strain of Streptomyces erythreus and hydroxylation at carbon 6 of the erythranolide ring system by a soluble noninduced cell-free enzyme system. Biochemistry 21:263–269
Frear DS, Swanson HR, Tanaka FS (1969) N-Demethylation of substituted 3-(phenyl)-l-methylureas: isolation and characterization of a microsomal mixed function oxidase from cotton. Phytochemistry 8:2157–2169
Jefcoate CR (1978) Measurement of substrate and inhibitor binding to microsomal cytochrome P-450 by optical difference spectroscopy. In: Fleisher S, Packer L (eds) Methods in enzymology, vol LII. Academic Press, New York, pp 258–279
Kotake AN, Funae Y (1980) High performance liquid chromatographic technique for resolving multiple forms of hepatic membrane bound cytochrome P-450. Proc Natl Acad Sci USA 77:6473–6475
Leto KJ (1984) Functional discrimination between photosystem II-associated chlorophyll a proteins in Zea mays. Biochim Biophys Acta 766:98–108
Omura R, Sato R (1964) The carbon monoxide binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239:2370–2378
Peterson JA, Prough RA (1986) Cytochrome P-450 reductase and cytochrome b5 in cytochrome P-450 analysis. In: Ortiz de Montellano PR (ed) Cytochrome P-450, structure, function, mechanism and biochemistry. Plenum Press, New York London, pp 89–117
Romesser JA, O'Keefe DP (1986) Induction of Cytochrome P-450 dependent sulfonylurea metabolism in Streptomyces griseolus. Biochem Biophys Res Commun 140:650–659
Sariaslani FS, Kunz DA (1986) Induction of cytochrome P-450 in Streptomyces griseus by soybean flour. Biochem Biophys Res Commun 141:405–410
Sutherland JB (1986) Demethylation of veratrole by cytochrome P-450 in Streptomyces setonii. Appl Environ Microbiol 52:98–100
Sweetser PB (1985) Safening of sulfonylurea herbicides to cereal crops: mode of herbicide antidote action. Proceedings 1985 British Crop Protection Conf, pp 1147–1154
Sweetser PB, Schow GS, Hutchison JM (1982) Metabolism of chlorosulfuron by plants: Biological basis for selectivity of a new herbicide for cereals. Pestic Biochem Physiol 17:18–23
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O'Keefe, D.P., Romesser, J.A. & Leto, K.J. Identification of constitutive and herbicide inducible cytochromes P-450 in Streptomyces griseolus . Arch. Microbiol. 149, 406–412 (1988). https://doi.org/10.1007/BF00425579
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DOI: https://doi.org/10.1007/BF00425579