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Location and nucleotide sequence of the gene for cytochrome f in wheat chloroplast DNA

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Summary

The gene for cytochrome f has been located in wheat chloroplast DNA by hybridisation with a 3.3 kbp BglII fragment of pea chloroplast DNA containing the gene for cytochrome f, by in vitro transcription-translation of cloned restriction fragments of wheat chloroplast DNA and by nucleotide sequence analysis. The gene is located 3 kbp from the 3′ end of the gene for the large subunit of ribulose bisphosphate carboxylase and is transcribed from the same DNA strand. Nucleotide sequence analysis reveals an open reading frame of 320 amino acids, of which 285 amino acid residues comprise the mature polypeptide and 35 amino acid residues probably represent an N-terminal signal sequence. The nucleotide sequence of the wheat gene shows 85% homology with the gene for pea cytochrome f.

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Authors and Affiliations

  1. Department of Botany, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    David L. Willey, Christopher J. Howe & John C. Gray

  2. Protein Sequencing Unit, Department of Biochemistry, University of Leeds, LS2 9JI, Leeds, UK

    Anthony D. Auffret

  3. Plant Breeding Institute, Maris Lane, Trumpington, CB2 2LQ, Cambridge, UK

    Catherine M. Bowman & Tristan A. Dyer

Authors
  1. David L. Willey
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  2. Christopher J. Howe
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  3. Anthony D. Auffret
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  4. Catherine M. Bowman
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  5. Tristan A. Dyer
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  6. John C. Gray
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Communicated by H. Saedler

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Willey, D.L., Howe, C.J., Auffret, A.D. et al. Location and nucleotide sequence of the gene for cytochrome f in wheat chloroplast DNA. Molec. Gen. Genet. 194, 416–422 (1984). https://doi.org/10.1007/BF00425553

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  • DOI: https://doi.org/10.1007/BF00425553

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