Abstract
Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rhamnose, galactose, 3-amino-3,6-dideoxygalactose, an unknown amino sugar and galacturonic acid, it represents very likely the capsule of R. capsulata Sp11.
Polysaccharide I has a serological specificity different from that of polysaccharide II as shown by immunoprecipitation using antisera against living cells. Polysaccharide II, but not polysaccharide I, reacts in antiserum against heattreated cells (100°C, 2.5 h). Whole cells are agglutinated in the antisera against living but not in those against heattreated cells.
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References
Costerton JW, Irwin RT, Cheng KJ (1981) The bacterial glycocalyx in nature and disease. Annu Rev Microbiol 35:299–324
Dixon JS, Lipkin D (1954) Spectrophotometric determination of vicinal glycols application to the determination of ribofuranosides. Anal Chem 26:1092–1093
Hirschfeld J (1960) Immunoelectrophoretic procedure and application to the study of group-specific variations in sera. Science tools, vol 7. Stockholm: LKB 1960, pp 18–25
Jann K, Westphal O (1975) Microbial polysaccharides. In: Sela M (ed) The antigens, vol III. Academic Press, New York, pp 1–125
Markovitz A (1977) Genetics and regulation of bacterial capsular polysaccharide. Biosynthesis and radiation sensitivity. In: Sutherland J (ed) Surface carbohydrates of the prokaryotic cell. Academic Press, New York, pp 415–462
Oelze J, Golecki JR, Kleinig H, Weckesser J (1975) Characterization of two cell envelope fractions from chemotrophically grown Rhodospirillum rubrum. Antonie van Leeuwenhoek, J Microbiol Serol 41:273–286
Omar AS, Flammann HT, Golecki JR, Weckesser J (1983a) Detection of capsule and slime polysaccharide layers in two strains of Rhodopseudomonas capsulata. Arch Microbiol 134: 114–117
Omar AS, Flammann HT, Borowiak D, Weckesser J (1983b) Lipopolysaccharides of two strains of the phototrophic bacterium Rhodopseudomonas capsulata. Arch Microbiol 134: 212–216
Ørskov I, Ørskov F, Jann B, Jann K (1977) Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev 41:667–710
Ouchterlony O (1965) Gel-diffusion techniques. Immunchemie 15. Coll., Mosbach. Springer, Berlin Heidelberg New York
Scheidegger JJ (1955) Une microméthode de l'immunoélectrophorèse. Int Arch Allergy 7:103–110
Schrader M, Drews G, Weckesser J, Mayer H (1982) Polysaccharide containing 6-O-methyl-d-mannose in Chlorogloeopsis PCC 6912. J Gen Microbiol 128:273–277
Sutherland JW (1977) Surface carbohydrates of the prokaryotic cell. In: Sutherland IW (ed) Bacterial exopolysaccharides. Their nature and production. Academic Press, New York, pp 27–69
Westphal O, Jann K (1965) Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods in Carbohydr Chem 5:83–91
Westphal O, Lüderitz O, Bister F (1952) Über die Extraktion von Bakterien mit Phenol/Wasser. Z Naturforsch 7b:148–155
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Omar, A.S., Weckesser, J. & Mayer, H. Different polysaccharides in the external layers (capsule and slime) of the cell envelope of Rhodopseudomonas capsulata Sp11. Arch. Microbiol. 136, 291–296 (1983). https://doi.org/10.1007/BF00425219
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DOI: https://doi.org/10.1007/BF00425219