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Chemoautotrophic growth of a marine Beggiatoa in sulfide-gradient cultures

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Abstract

A recently isolated strain of Beggiatoa, MS-81-6 (cf. alba), was tested for chemoautotrophic growth in semi-solid (0.2% agar) sulfide/oxygen gradient cultures. The organism grew in a horizontal layer, the distance from the air/medium interface depending on sulfide concentrations and changing with time. Optimal growth as a gradient organism was based on a preference for reduced oxygen concentrations and a limited sulfide tolerance in combination with gliding motility. In gradient cultures chemoautotrophic growth was demonstrated by the following criteria: (1) biomass yield (protein) increased with increasing sulfide concentration, and estimated molar growth yields agreed with those for other sulfide-grown chemoautotrophs; (2) approximately 90% of total cell carbon and protein carbon were fixed from carbon dioxide; (3) the CO2-fixing enzyme, ribulosebisphosphate carboxylase, was present in cell-free extracts at a level typical of chemoautotrophs; (4) acidification of the medium, apparently linked to utilization of internal So granules, accompanied the later phase of growth. The ability to grow on acetate in the absence of a source of reduced sulfur renders the organism facultatively chemoautotrophic.

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Abbreviations

RuBP:

d-Ribulose-1,5-bisphosphate

RuBP-carboxylase:

d-Ribulose-1,5-bisphosphate carboxylase (E.C. 4.1.1.39)

So :

elemental sulfur

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Department of Biology, Woods Hole Oceanographic Institution, 02543, Woods Hole, MA, USA

    Douglas C. Nelson & Holger W. Jannasch

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  1. Douglas C. Nelson
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  2. Holger W. Jannasch
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Nelson, D.C., Jannasch, H.W. Chemoautotrophic growth of a marine Beggiatoa in sulfide-gradient cultures. Arch. Microbiol. 136, 262–269 (1983). https://doi.org/10.1007/BF00425214

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  • DOI: https://doi.org/10.1007/BF00425214

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