Skip to main content
SpringerLink
Log in

Licht-und elektronenmikroskopische Untersuchungen an meiotischen Basidien von Coprinus radiatus (Bolt) Fr.

Light and electron microscopic studies of meiotic basidia of Coprinus radiatus (Bolt) Fr.

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

The basidia of Coprinus radiatus develop synchronously. Both meiotic divisions happen within 30–40 min. During this time an undifferentiated centriole appears, divides and forms the spindle poles by radiating microtubules. For the nuclear envelope breaks down in the metaphase and restituates during telophase the meiosis is not intranuclear.

In meiotic basidia ER is regularly to be seen near the cell wall, it may be compared with the “ascus vesicle”. Moreover an organized ER-complex occurs during prophase. Its function is supposed to be a pool for restituation of the nuclear envelope; therefore it may be called “Kernteilungs-Reticulum”.

Zusammenfassung

Bei coprinus radiatus entwickeln sich die Basidien synchron. Die beiden meiotischen Teilungen laufen innerhalb von 30–40 min ab. Dabei erscheint ein undifferenziertes Centriol, das sich teilt und unter Ausstrahlung von Mikrotubuli die Spindelpole aufbaut. Die Kernhülle fragmentiert während der Metaphase und wird in der Telophase restauriert; es handelt sich hier also nicht um einen intranucleären Vorgang.

In den meiotischen Basidien läßt sich regelmäßig wandständiges ER nachweisen, das man mit dem “Ascusvesicel” vergleichen kann. Außerdem wird zum Zeitpunkt der Prophase ein organisierter ER-Komplex gebildet, dessen Funktion vermutlich darin besteht, als Reservoir für die Restitution der Kernhülle zu dienen; er soll deshalb als “Kernteilungs-Reticulum” bezeichnet werden.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Aist, J. R.: The mitotic apparatus in fungi, Ceratocystis fagacearum and Fusarium oxysporum. J. Cell Biol. 40, 120–135 (1969).

    Google Scholar 

  • Aldrich, H. C.: The ultrastructure of meiosis in three species of Physarum. Mycologia (N. Y.) 59, 127–148 (1967).

    Google Scholar 

  • —: The ultrastructure of mitosis in myxamoebae and plasmodia of Physarum flavicomum. Amer. J. Bot. 56, 290–299 (1969).

    Google Scholar 

  • Bajer, A., Mole-Bajer, J.: Formation of spindle fibers, kinetochore orientation and behavior of the nuclear envelope during mitosis in endosperm. Fine structural and in vitro studies. Chromosoma (Berl.) 27, 448–484 (1969).

    Google Scholar 

  • Berlin, J. H., Bowen, C. C.: Centriols in the fungus Albugo candida. Amer. J. Bot. 51, 650–652 (1964).

    Google Scholar 

  • ——: Mitosis and zoospore formation in Albugo. Amer. J. Bot. 52, 613 (1965).

    Google Scholar 

  • Berliner, M. D., Duff, R. H.: Ultrastructure of Armillaria mellea hyphae. Canad. J. Bot. 43, 171–172 (1965).

    Google Scholar 

  • Bouck, G. B., Cronshaw, J.: The fine structure of differentiating sieve tube elements. J. Cell Biol. 25, 79–95 (1965).

    Google Scholar 

  • Bracker, C. E.: Ultrastructure of fungi. Ann. Rev. Phytopath. 5, 343–374 (1967).

    Google Scholar 

  • Burnett, J. H.: Fundamentals of mycology. London: Edw. Arnold 1968.

    Google Scholar 

  • Dowding, E. S., Bakerspigel, A.: The migrating nucleus. Canad. J. Microbiol. 1, 68–78 (1954).

    Google Scholar 

  • Drawert, H., Rüffer-Bock, U.: Fluorochromierung von endoplasmatischem Reticulum, Dictyosomen und Chondriosomen mit Tetracyclin. Ber. dtsch. bot. Ges. 77, 440–449 (1964).

    Google Scholar 

  • Esser, K., Kuenen, R.: Genetik der Pilze. Berlin-Heidelberg-New York: Springer 1965.

    Google Scholar 

  • Falk, H., Kleinig, H.: Feinbau und Carotinoide von Tribonema (Xanthophyceae). Arch. Mikrobiol. 61, 347–362 (1968).

    Google Scholar 

  • Fawcett, D. W.: An atlas of fine structure: the cell, its organelles and inclusions. Philadelphia-London: W. B. Saunders 1966.

    Google Scholar 

  • —, Revel, J. P.: The sarcoplasmic reticulum of a fast-acting fish muscle. J. biophys. biochem. Cytol. 10, 89–110 Suppl. (1961).

    Google Scholar 

  • Fuller, M. S., Reichle, R.: The zoospore and early development of Rhizidiomyces apophysatus. Mycologia (N. Y.) 57, 946–961 (1965).

    Google Scholar 

  • Girbardt, M.: Licht-und elektronenoptische Untersuchungen an Polystictus versicolor (L). VIII. Färberische Analyse der vegetativen Kernteilung. Planta (Berl.) 58, 1–21 (1962).

    Google Scholar 

  • —: Submikroskopische Cytologie der Pilzzelle. Fortschr. Bot. 29, 25–32 (1967).

    Google Scholar 

  • —: Ultrastructure and dynamics of the moving nucleus. Aspects of cell motility. XXIInd Sympos. Soc. Exp. Biol. Oxford, pp. 249–259. Cambridge: Univ. Press 1968.

    Google Scholar 

  • Greenhalgh, G. N., Griffiths, H. B.: The ascus vesicle. Trans. Brit. mycol. Soc. 54, 489–492 (1970).

    Google Scholar 

  • Hafner, L., Thielke, Ch.: Kernzahl und Zellgröße im Fruchtkörperstiel von Coprinus radiatus (Bolt) FR. Ber. dtsch. bot. Ges. 83, 27–31 (1970).

    Google Scholar 

  • Hawker, L. E.: Fine structure of fungi as revealed by electron microscopy. Biol. Rev. 40, 52–92 (1965).

    Google Scholar 

  • Hrushovetz, S. B.: Cytological studies of ascus development in Cochliobolus sativus. Canad. J. Bot. 34, 641–651 (1956).

    Google Scholar 

  • Ichida, A. A., Fuller, M. S.: Ultrastructure of mitosis in the aquatic fungus Catenaria anguillulae. Mycologia (N. Y.) 60, 141–155 (1968).

    Google Scholar 

  • Lerbs, V., Thielke, Ch.: Die Entstehung der Spindel während der Meiose von Coprinus radiatus. Arch. Mikrobiol. 68, 95–98 (1969).

    Google Scholar 

  • Lessie, P. E., Lovett, J. S.: Ultrastructural changes during sporangium formation and zoospore differentiation in Blastocladiella emersonii. Amer. J. Bot. 55, 220–236 (1968).

    Google Scholar 

  • Lowry, R. J., Sussman, A. S.: Ultrastructural changes during germination of ascospores of Neurospora tetrasperma. J. gen. Microbiol. 51, 403–409 (1968).

    Google Scholar 

  • Lowry, R. J., Sussman, A. S.: Ultrastructural changes during germination of ascospores of Neurospora tetrasperma. J. gen. Microbiol. 51, 403–409 (1968).

    Google Scholar 

  • Lu, B. C.: Golgi apparatus of the basidiomycete Coprinus lagopus. J. Bact. 92, 1831–1834 (1966a).

    Google Scholar 

  • —: Fine structure of meiotic chromosomes of the basidiomycete Coprinus lagopus. Exp. Cell Res. 43, 224–227 (1966b).

    Google Scholar 

  • —: Meiosis in Coprinus lagopus: a comparative study with light and electron microscopy. J. Cell Sci. 2, 529–536 (1967).

    Google Scholar 

  • Luft, J. H.: Permanganate—a new fixative for electron microscopy. J. biophys. biochem. Cytol. 2, 799–801 (1956).

    Google Scholar 

  • Manton, J.: Observations with electron-microscope on the division cycle in the flagellate Prymnesium parvum Carter. J. roy. micr. Soc. 83, 317–322 (1964).

    Google Scholar 

  • Moor, H.: Ultrastrukturen im Zellkern der Bäckerhefe. J. Cell Biol. 29, 153–155 (1966).

    Google Scholar 

  • Moore, R. T.: Fine structure of mycota. 6. Occurrence of the Golgi dictyosome in the heterobasidiomycete Puccinia podophylli. J. Bact. 86, 866–871 (1963).

    Google Scholar 

  • —: The ultrastructure of fungal cells. In: The fungi. Vol. I, pp. 95–118 (eds. G. C. Ainsworth and A. S. Sussman). New York: Academic Press 1965.

    Google Scholar 

  • Moser, M.: Basidiomyceten II. In: Kleine Kryptogamenflora (ed. H. Gams). Stuttgart: G. Fischer 1967.

    Google Scholar 

  • Motta, J. J.: Somatic nuclear division in Armillaria mellea. Mycologia (N. Y.) 61, 873–886 (1969).

    Google Scholar 

  • Olive, L. S.: The structure and behavior of fungus nuclei. bot. Rev. 19, 439–586 (1953).

    Google Scholar 

  • Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285 (1952).

    Google Scholar 

  • Porter, K. R., Machado, R. D.: Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tip. J. biophys. biochem. Cytol. 7, 167–181 (1967).

    Google Scholar 

  • Prusso, D. C., Wells, K.: Sporobolomyces roseus. I. Ultrastructure. Mycologia (N.Y.) 59, 337–348 (1967).

    Google Scholar 

  • Reichle, R. E., Fuller, M. S.: The fine structure of Blastocladiella emersonii zoospores. Amer. J. Bot. 54, 81–92 (1967).

    Google Scholar 

  • Renaud, F. L., Swift, H.: The development of basal bodies and flagella in Allomyces arbusculus. J. Cell Biol. 23, 339–354 (1964).

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrat at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).

    Google Scholar 

  • Robinow, C. F.: Nuclear apparatus and cell structure of rod shaped bacteria. In: The bacterial cell (ed. R. J. Dubos). Cambridge, Mass.: Havard Univ Press 1949.

    Google Scholar 

  • —, Bakerspigel, A.: Somatic nuclei and forms of mitosis in fungi. In: The fungi. Vol. I, pp. 119–142 (eds. G. C. Ainsworth and A. S. Sussman). New York: Academic Press 1965.

    Google Scholar 

  • —, Marak, J.: A fiber apparatus in the nucleus of the yeast cell. J. Cell Biol. 29, 129–151 (1966).

    Google Scholar 

  • Roth, L. E., Wilson, H. J., Chakraborty, J.: Anaphase structure in mitotic cells typified by spindle elongation. J. Ultrastruct. Res. 14, 460–483 (1966).

    Google Scholar 

  • Sabatini, D. D., Bensch, K. G., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyd fixation. J. Cell Biol. 17, 19–58 (1963).

    Google Scholar 

  • Schrantz, J.: Contribution à l'étude de la formation de la paroi sporale chez Pustularia cupularis (L) Fuck. C. R. Acad. Sci. (Paris) 262, 1212–1215 (1966).

    Google Scholar 

  • —: Présence d'un aster, cours des mitoses de l'asque et de la formation des ascospores chez l'Ascomycète Pustularia cupularis (L) Fuck. C. R. Acad. Sci. (Paris) 264, 1274–1277 (1967).

    Google Scholar 

  • —: Étude cytologique, en microscopie optique et électronique, de quelques Ascomycètes. I. Le noyau. Rev. Cytol. Biol. végét. 33, 1–100 (1970).

    Google Scholar 

  • Thielke, Ch.: Membransysteme in meiotischen Basidien. Ber. dtsch. bot. Ges. 81, 183–186 (1968a).

    Google Scholar 

  • —: Restitution der Kernmembran in postmeiotischen Basidien. Ber. dtsch. bot. Ges. 81, 315–316 (1968b).

    Google Scholar 

  • Venable, J. H., Coggeshall, R.: A simplified lead citrat stain for use in electron microscopy. J. Cell Biol. 25, 407–412 (1965).

    Google Scholar 

  • Wells, K.: Light and electron microscopic studies of Ascobolus stercorarius. I. Nuclear division in the ascus. Mycologia (N.Y.) 62, 761–790 (1970).

    Google Scholar 

  • Wilson, C. L., Aist, J. R.: Motility of fungal nuclei. Phytopath. 57, 769–771 (1967).

    Google Scholar 

  • Wohlfarth-Bottermann, K. E.: Die Kontrastierung tierischer Zellen und Gewebe im Rahmen ihrer elektronenmikroskopischen Untersuchung an ultradünnen Schnitten. Naturwissenschaften 44, 287–288 (1957).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pflanzenphysiologisches Institut der Freien Universität Berlin, Berlin, Deutschland

    V. Lerbs

  2. Abteilung für Elektronenmikroskopie der Freien Universität Berlin, Berlin, Deutschland

    V. Lerbs

Authors
  1. V. Lerbs
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Kurzfassung einer Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Freien Universität Berlin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lerbs, V. Licht-und elektronenmikroskopische Untersuchungen an meiotischen Basidien von Coprinus radiatus (Bolt) Fr.. Archiv. Mikrobiol. 77, 308–330 (1971). https://doi.org/10.1007/BF00425034

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00425034

Search

Navigation