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Growth of wild type and spreading colonial mutants of Neurospora crassa in batch culture and on agar medium

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Summary

  1. 1.

    The colony radial growth rates (K r) of wild type and spreading colonial mutants (spco) of Neurospora crassa were found to be a function of the strain's specific growth rate (α) and the width (w) of the colony's peripheral growth zone.

    $$K_\tau = \alpha w$$
  2. 2.

    Except for one wild type strain and a temperature sensitive mutant (cot 3), all the 20 strains studied had approximately the same maximum specific growth rate.

  3. 3.

    The width of the peripheral growth zones of the strains varied from 493 to 6800 μm.

  4. 4.

    There was a direct relationship between the colony radial growth rates of the strains and the diameter and hyphal compartment (apical and intercalary) length of their leading hyphae.

  5. 5.

    The radial growth rate of spco 4 colonies increased linearly with medium depth.

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References

  • Brody, S., Tatum, E. L.: The primary biochemical effect of a morphological mutation in Neurospora crassa. Proc. nat. Acad. Sci. (Wash.). 56, 1290–1297 (1966).

    Google Scholar 

  • Brody, S., Tatum, E. L.: Phosphoglucomutase mutants and morphological changes in Neurospora crassa. Proc. nat. Acad. Sci. (Wash.) 58, 923–930 (1967).

    Google Scholar 

  • Butler, G. M.: Growth of hyphal branching systems in Coprinus disseminatus. Ann. Bot. N. S. 25, 341–352 (1961).

    Google Scholar 

  • Clutterbuck, A. J., Roper, J. A.: A direct determination of nuclear distribution in heterokaryons of Aspergillus nidulans. Genet. Res. 7, 185–194 (1966).

    Google Scholar 

  • Garnjobst, L., Tatum, E. L.: A survey of new morphological mutants in Neurospora crassa. Genetics 57, 579–604 (1967).

    Google Scholar 

  • Lechner, J. F., Fuscaldo, K. E.: Studies on colonial mutants of Neurospora crassa. Bact. Proc. 1968, 143.

  • Lhoas, P.: Growth rate and haploidization of Aspergillus niger on medium containing p-fluorophenylalanine. Genet. Res. 12, 305–315 (1968).

    Google Scholar 

  • Mishra, N. C., Tatum, E. L.: Phosphoglucomutase mutants of Neurospora sitophila and their relation to morphology. Proc. nat. Acad. Sci. (Wash.) 66, 638–645 (1970).

    Google Scholar 

  • Pirt, S. J.: A kinetic study of the mode of growth of surface colonies of bacteria and fungi. J. gen. Microbiol. 47, 181–197 (1967).

    Google Scholar 

  • Reissing, J. L., Glasgow, J. E.: Mucopolysaccharide which regulates growth in Neurospora. J. Bact. 106, 882–889 (1971).

    Google Scholar 

  • Ryan, F. J., Beadle, G. W., Tatum, E. L.: The tube method of measuring the growth rate of Neurospora. Amer J. Bot. 30, 789–799 (1943).

    Google Scholar 

  • Scott, W. A., Tatum, E. L.: Glucose-6-phosphate dehydrogenase and Neurospora morphology. Proc. nat. Acad. Sci. (Wash.) 66, 515–522 (1970).

    Google Scholar 

  • Trinci, A. P. J.: A kinetic study of the growth of Aspergillus nidulans and other fungi. J. gen. Microbiol. 57, 11–24 (1969).

    Google Scholar 

  • Trinci, A. P. J.: Kinetics of the growth of mycelial pellets of Aspergillus nidulans. Arch. Mikrobiol. 73, 353–367 (1970).

    Google Scholar 

  • Trinci, A. P. J.: Influence of the peripheral growth zone on the radial growth rate of fungal colonies. J. gen. Microbiol. 67, 325–344 (1971).

    Google Scholar 

  • Trinci, A. P. J.: Culture turbidity as a parameter of mould growth. Trans. Brit. mycol. Soc. 58, 467–473 (1972).

    Google Scholar 

  • Trinci, A. P. J.: The hyphal growth unit of wild type and spreading colonial mutants of Neurospora crassa. Arch. Mikrobiol. 91, 127–136 (1973).

    Google Scholar 

  • Trinci, A. P. J., Collinge, A. J.: Structure and plugging of septa of wild type and spreading colonial mutants of Neurospora crassa. Arch. Mikrobiol. (in press, 1973).

  • Vogel, H. J.: A convenient growth medium for Neurospora (medium N). Microbiol. Genet. Bull. 13, 42 (1956).

    Google Scholar 

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Trinci, A.P.J. Growth of wild type and spreading colonial mutants of Neurospora crassa in batch culture and on agar medium. Archiv. Mikrobiol. 91, 113–126 (1973). https://doi.org/10.1007/BF00424755

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