Abstract
Four isolates of a gram-negative flexible bacterium have been obtained from brine water samples of the Atlantis II Deep of the Red Sea at a depth of 2000 m. One isolate (MAS 10) was studied in detail. Cells are nonmotile, flexible rods, measuring about 0.3 μm in width and 5 to 50 μm in length. The new organisms are heterotrophs growing anaerobically on yeast extract, meat extract, peptone, tryptone, and, less efficiently, on acetate and casamino acids. Growth occurs between 30% and 53°C at pH 6 to 8 in the presence of at least 3% NaCl. The shortest doubling time is 8.5 h under optimal growth conditions. Cells are sensitive to the antibiotics penicillin, ampicillin, vancomycin, and streptomycin, but resistant to tetracyclin and rifamipicin. The GC-content of the DNA is 39 mol%. Based on their 16S rRNA the new isolates group with the general cluster of eubacterial phyla. Since they show no specific relationship to any of them, a new genus is described, which is named Flexistipes, the flexible stick. Type species is Flexistipes sinusarabici strain MAS 10 (DSM 4947).
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References
Bäcker H (1973) Rezente hydrothermal-sedimentäre Lagerstättenbildung. Erzmetall 26: 544–555
Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32: 781–791
Bergmeyer HU (1974) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim
Biggin MD, Gibson TJ, Hong GF (1983) Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 80: 3963–3965
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911–917
Brewer PG, Densmore CD, Munns R, Stanley RJ (1969) Hydrography of the Red Sea brines. In: Degens ET, Ross DA (eds) Hot brines and recent heavy metal deposits in the Red Sea. Springer, Berlin Heidelberg New York, pp 138–147
Brosius J, Palmer JL, Kennedy JP, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci USA 75: 4801–4805
Degens ET, Ross DA (1969) Hotbrines and recent heavymetal deposits in the Red Sea. Springer, Berlin Heidelberg New York.
De Soete G (1983) A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48: 621–626
Dowler MJ, Ingmanson DE (1979) Thiocyanate in Red Sea brine and its implications. Nature 279: 51–52
Fiala G, Stetter KO (1986) Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebactria growing optimally at 100° C. Arch Microbiol 145: 56–61
Fiala G, Stetter KO, Jannasch HW, Langworthy TA, Madon J (1986) Staphylothermus marinus sp. nov. represents a novel genus of extremely thermophilic submarine heterotrophic archaebacteria growing up to 98° C. Syst Appl Microbiol 8: 106–113
Hartmann M (1985) Atlantis II deep geochemical brine system. Chemical processes between hydrothermal brines and Red Sea deep water. Mar Geol 64: 157–177
Hilpert R, Winter J, Hammes W, Kandler O (1981) The sensitivity of archaebacteria to antibiotics. Zentralbl Bakteriol Hyg I Abt Orig C2: 11–20
Huber R, Langworthy TA, König H, Thomm M, Woese CR, Sleytr UB, Stetter KO (1986) Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90° C. Arch Microbiol 144: 324–333
Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic Press, New York, pp 21–132
Kafatos FC, Jones CW, Efstratiadis A (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucl Acids Res 1: 1541–1552
Kandler O (1962) Cell wall structures and their phylogenetic implications. Zentralbl Bakteriol Hyg I Abt Orig C 3: 149–160
König H, Stetter KO (1982) Isolation and characterization of Methanolobus tindarius sp. nov., a coccoid methanogen growing only on methanol and methylamines. Zentralbl Bakteriol Hyg I Abt Orig C 3: 478–490
Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR (1985) Rapid determination of 16S ribosomal RNA sequences for phylogentic analysis. Proc Natl Acad Sci USA 82: 6955–6959
Langworthy TA (1982) Lipids of Thermoplasma Methods Enzymol 88: 396–406
Langworthy TA, Holzer G, Zeikus JG, Tornabene TG (1983) Isoand anteiso-branched glycerol diethers of the thermophilic anaerobe Thermodesulfotobacterium commune. Syst Appl Microbiol 4: 1–17
Lauerer G, Kristjansson JK, Langworthy TA, König H, Stetter KO (1986) Methanothermus sociabilis sp. nov., a second species within the Methanothermaceae growing at 97° C. Syst Appl Microbiol 8: 100–105
Marmur J Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118
Möller B, Ossmer R, Howard BH, Gottschalk G, Hippe H (1984) Sporomusa, a new genus of gram-negative, anaerobic bacteria including, Sporomusa sphaeroides spec. nov., and Sporomusa ovata spec. nov. Arch Microbiol 139: 388–396
Murray CL, Rabinowitz JC (1981) RNA polymerase from Clostridium acidi-urici. J Biol Chem 256: 5153–5161
Oren A, Weisburg WG, Kessel M, Woese CR (1984) Halobacteroides halobius gen. nov., sp. nov., a moderately halophilic anaerobic bacterium from the bottom sediments of the Dead Sea. Syst Appl Microbiol 5: 58–70
Oren A, Pohla A, Stackebrandt E (1987) Transfer of Clostridium lortetii to a new genus Sporohalobacter gen. nov. as Sporohalobacter lortetii comb. nov., and description of Sporohalobacter marismortui sp. nov. Syst Appl Microbiol 9: 239–246
Petzold M (1987) Hot brine structure and bacteria. In: Theil (ed) Cruis report: Meteor Cruise 5, leg 2. University Hamburg
Rengpipat S, Langworthy TA, Zeikus JG (1988) Halobacteroides acetoethylicus sp. nov., a new obligately anaerobic halophile isolated from deep subsurface hypersaline environments. Syst Appl Microbiol 11 28–35
Rhuland LE, Work E, Denman RF, Hoare DS (1955) The behaviour of the isomers of α,ε-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77: 4844–4846
Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev 36: 407–477
Watson SW, Waterbury JB (1969) Sterile hot brines of the Red Sea. In: Degens ET, Ross DA (eds) Hot brines and recent heavy metal deposits in the Red Sea. Springer, Berlin Heidelberg New York, pp 272–281
Weisburg WG, Tully JG, Rose DL, Petzel JP, Oyaizu H, Yang D, Mandelco L, Sechrest J, Lawrence TG, Van Etten J, Maniloff J, Woese CR (1989) A phylogentic analysis of the Mycoplasmas basis for their classification. J Bacteriol 171: 6455–6467
Williams WJ (1979) Handbook of anion determination. Butterworths, London, pp 570–572
Woese CR (1987) Bacterial evolution. Microbiol Rev 51: 227–271
Woese CR, Sogin ML, Stahl DA, Lewis BJ, Bonen L (1976) A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli. J Mol Evol 7: 197–213
Woese CR, Gutell R, Gupta R, Noller HF (1983) Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol Rev 47: 621–669
Zeikus JG Hegge PW, Thompson TE, Phelps TJ, Langworthy TA (1983) Isolation and characterization of Haloanaerobium praevalens gen. nov. and sp. nov., and obligately anaerobic halophile common to Great Salt Lake sediments. Curr Microbiol 9: 225–234
Zillig W, Stetter KO, Schnabel R, Thomm M (1985) DNA-dependent RNA polymerases of the archaebacteria. In: Woese CR, Wolfe RS (eds) The bacteria, vol 8: Archarbacteria. Academic Press, New York, pp 499–521
ZoBell CE (1941) Studies on marine bacteria. I. The cultural requirements of heterotrophic anaerobes J Mar Res 4: 42–75
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Fiala, G., Woese, C.R., Langworthy, T.A. et al. Flexistipes sinusarabici, a novel genus and species of eubacteria occurring in the Atlantis II Deep brines of the Red Sea. Arch. Microbiol. 154, 120–126 (1990). https://doi.org/10.1007/BF00423320
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DOI: https://doi.org/10.1007/BF00423320