Summary
Normal dogs were injected i.V. with a single dose of 0.25 mg/kg sodium salt of HB 419 (Glibenclamide). Plasma insulin and glucose concentrations were measured at stated intervals over a period of two hours. The rise in insulin, but not the hypoglycemic response was abolished in peripheral blood when the animals were pretreated with a single i.v. injection of either 0.1 mg/kg or 0.3 mg/kg dl-propranolol, 30 min prior to the administration of HB 419. The d-isomer of propranolol was ineffective in this respect. These results indicate that a) the mechanism by which propranolol inhibits sulphonylurea-stimulated insulin secretion involves beta adrenergic receptors; and b) the hypoglycemia produced by HB 419 in the presence of propranolol could be the result of extrapancreatic effects. Since the possibility of an early rise in the insulin concentration of portal blood was not excluded in our present series of experiments, final proof will have to be provided by studies in totally pancreatectomized dogs.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Sirek, O. V., Vigas, M., Niki, A., Niki, H., Sirek, A.: Betaadrenergic stimulation and insulin release in dogs following HB 419. Diabetologia 5, 207–210 (1969)
Sirek, A., Sirek, O. V., Smigura, F., Przybylska, K.: Effect of adrenergic blocking agents on the acute tolbutamide response in dogs. Acta diabet. lat. 7, 777–788 (1970)
Nickerson, M.: Drugs inhibiting adrenergic nerves and structures innervated by them. II. β-adrenergic blocking agents. In: The pharmaceutical basis of therapeutics. 4th Edition, (eds. L. S. Goodman, A. Gilman) pp. 565–570. New York: The Macmillan Co. 1970
Hales, C. N., Randle, P. J.: Immunoassay of insulin with insulin-antibody precipitate. Lancet 1963 I, 200
Hales, C. N., Randle, P. J.: Immunoassay of insulin with insulin-antibody precipitate. Biochem. J. 88, 137–146 (1963)
Huffman, W. S.: A rapid photoelectric method for the determination of glucose in blood and urine. J. biol. Chem. 120, 51–55 (1937)
Houssay, B. A., Penhos, J. C., Urgoiti, E., Teodosio, N., Apelbaum, J. Bowkett, J.: The role of insulin in the action of the hypoglycemic sulfonyl compounds. Ann. N.Y. Acad. Sci. 71, 25–34 (1957)
Loubatières, A., Bouyard, P., Fruteau de Laclos, C., Sassine, A., Alric, R.: Renforcement et prolongation des effects de l'insuline par les sulfonamidés hypoglycémiants et antidiabétiques. C.R. Soc. Biol. (Paris) 150, 1601–1603 (1956)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sirek, O.V., Sirek, A. & Policova, Z. Inhibition of sulphonylurea-stimulated insulin secretion by beta adrenergic blockade. Diabetologia 11, 269–272 (1975). https://doi.org/10.1007/BF00422390
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00422390