Skip to main content
SpringerLink
Log in

Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Zusammenfassung

Es wird ein Verfahren zur Submerskultur von Knallgasbakterien beschrieben. Es beruht auf der kräftigen Magnetrührung der Nährlösung unter einem Gemisch von H2, O2 und CO2. Der hohen O2-Empfindlichkeit der Zellen wird durch „Gradientenbegasung” Rechnung getragen.

Der fakultativ chemolithotrophe Hydrogenomonas-Stamm 20 wurde bakteriologisch charakterisiert und wachstumsphysiologisch untersucht.

Die Generationszeit beträgt während der log-Phase 21/6 Std, die scheinbare Verdoppelungszeit 31/5 Std (28° C).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literatur

  • Atkinson, D. E., and B. A. McFadden: The biochemistry of Hydrogenomonas. I. The Hydrogenase of Hydrogenomonas facilis in cell-free preparations. J. biol. Chem. 210, 885–893 (1954).

    Google Scholar 

  • Atkinson, D. E.: The Biochemistry of Hydrogenomonas. III. The effect of inorganic nitrogen compounds on hydrogen uptake. J. Bact. 70, 78 (1955).

    Google Scholar 

  • Atkinson, D. E.: The biochemistry of Hydrogenomonas. IV. The inhibition of hydrogenase by oxygen. J. biol. Chem. 218, 557–564 (1956).

    Google Scholar 

  • Bergmann, F. H., J. C. Towne and R. H. Burris: Assimilation of carbon dioxide by hydrogen bacteria. J. biol. Chem. 230, 13–24 (1958).

    Google Scholar 

  • Cohen, J. S., and R. H. Burris: A method for the culture of hydrogen bacteria. J. Bact. 69, 316 (1955).

    Google Scholar 

  • Erb, W., u. W. Maurer: Biosynthese von 14C-markiertem Eiweiß mit Chlorella pyrenoidosa. Biochem. Z. 332, 388–395 (1960).

    Google Scholar 

  • Henrici, A. T.: Morphologic variation and the rate of growth of bacteria. London: Baillière, Tindall & Cox 1928.

    Google Scholar 

  • Hershey, A. D.: Factors limiting bacterial growth. IV. The age of the parent culture and the rate of growth of transplants of Escherichia coli. J. Bact. 37, 285–299 (1939).

    Google Scholar 

  • Kaserer, H.: Die Oxydation des Wasserstoffs durch Mikroorganismen. Zbl. Bakt., II. Abt. 16, 681 (1906).

    Google Scholar 

  • Kluyver, A. J., and A. Manten: Some observations on the metabolism of bacteria oxidizing molecular hydrogen. Antonie v. Leeuwenhoek 8, 71–86 (1942).

    Google Scholar 

  • La Rivière, J. W. M.: On the microbial metabolism of the tartaric acid isomers. Dissertation. Delft 1958.

  • Lebedeff, A. F.: Über die Assimilation des Kohlenstoffs bei wasserstoffoxydierenden Bakterien. Ber. dtsch. bot. Ges. 27, 598–602 (1909).

    Google Scholar 

  • Linday, M., and P. J. Syrett: The induced synthesis of hydrogenase by Hydrogenomonas facilis. J. gen. Microbiol. 19, 223–227 (1958).

    Google Scholar 

  • McFadden, B. A.: Some products of 14CO2-fixation by Hydrogenomonas facilis. J. Bact. 77, 339–343 (1959).

    Google Scholar 

  • Marino, R. J., and C. E. Clifton: Oxidative assimilation in suspensions and cultures of Hydrogenomonas facilis. J. Bact. 69, 188–192 (1955).

    Google Scholar 

  • Martin, D. S.: The oxygen consumption of Escherichia coli during the lag and logarithmic phases of growth. J. gen. Physiol. 15, 691–708 (1932).

    Google Scholar 

  • Newton, J. W., and J. B. Wilson: CO2 requirements and nucleic acid synthesis by Brucella abortus. J. Bact. 68, 74–76 (1954).

    Google Scholar 

  • Niel, C. B. van: The kinetics of growth of microorganisms. The chemistry and physiology of growth. Princeton Univ. Press 1949.

  • Niklewski, B.: Ein Beitrag zur Kenntnis wasserstoffoxydierender Mikroorganismen. II. Zbl. Bakt., II. Abt. 20, 469–473 (1908).

    Google Scholar 

  • Packer, L., and W. Vishniao: Chemosynthetic fixation of carbon dioxide and characteristics of hydrogenase in resting cell suspensions of Hydrogenomonas Ruhlandii nov. spec. J. Bact. 70, 216–223 (1955).

    Google Scholar 

  • Rahn, O., and G. L. Richardson: Oxygen demand and oxygen supply. J. Bact. 41, 225–249 (1941).

    Google Scholar 

  • Rahn, O., and G. L. Richardson: Oxygen demand and oxygen supply. J. Bact. 44, 321–332 (1942).

    Google Scholar 

  • Ruhland, W.: Aktivierung von Wasserstoff und CO2-Assimilation durch Bakterien. Ber. dtsch. bot. Ges. 40, 180–184 (1922).

    Google Scholar 

  • Ruhland, W.: Beiträge zur Physiologie der Knallgasbakterien. Jb. wiss. Bot. 63, 321–389 (1924).

    Google Scholar 

  • Schatz, A., and C. Bovell: Growth and hydrogenase activity of a new bacterium Hydrogenomonas facilis. J. Bact. 63, 87–98 (1952).

    Google Scholar 

  • Schlegel, H. G.: Physiologische Untersuchungen an wasserstoffoxydierenden Bakterien. Arch. Mikrobiol. 18, 362–390 (1953).

    Google Scholar 

  • Schlegel, H. G.: Untersuchungen über den Phosphatstoffwechsel der wasserstoff-oxydierenden Bakterien. Arch. Mikrobiol. 21, 127–155 (1954).

    Google Scholar 

  • Wilson, E., H. A. Stout, D. Powelson and H. Koffler: Comparative biochemistry of the hydrogen bacteria. J. Bact. 65, 283–287 (1953).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Deutschland

    H. G. Schlegel, H. Kaltwasser & G. Gottschalk

Authors
  1. H. G. Schlegel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Kaltwasser
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Gottschalk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schlegel, H.G., Kaltwasser, H. & Gottschalk, G. Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. Archiv. Mikrobiol. 38, 209–222 (1961). https://doi.org/10.1007/BF00422356

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00422356

Search

Navigation