Summary
After infection of sensitive cells in the absence of a helper phage, the satellite bacteriophage P4 enters a temporary phase of uncommitted replication followed by commitment to either the repressed-integrated condition or the derepressed-high copy number mode of replication. The transient phase and the stable plasmid condition differ from each other in the pattern of protein synthesis, in the rate of P4 DNA replication and in the expression of some gene functions. The regulatory condition characteristic of the P4 plasmid state affects a superinfecting genome, preventing the establishment of the P4 immune condition.
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References
Barrett KJ, Marsh ML, Calendar R (1976) Interactions between a satellite bacteriophage and its helper. J Mol Biol 106:683–707
Barrett KJ, Blinkova A, Arnold G (1983) The bacteriophage P4 α gene is the structural gene for bacteriophage P4-induced RNA polymerase. J Virol 48:157–169
Calendar R, Ljungquist E, Dehò G, Usher DC, Goldstein R, Youderian P, Sironi G, Six EW (1981) Lysogenization by satellite phage P4. Virology 113:20–38
Clark AJ, Margulis AD (1965) Isolation and characterization of recombination deficient mutants of E. coli K12. Proc Natl Acad Sci USA 53:451–459
Dehò G (1983) Circular genetic map of satellite bacteriophage P4. Virology 126:267–278
Dehò G, Ghisotti D, Alano P, Zangrossi S, Borrello MG, Sironi G (1984) Plasmid mode of propagation of the genetic element P4. J Mol Biol 178:191–207
Geisselsoder J, Youderian P, Dehò G, Chidambaram M, Goldstein R, Ljungquist E (1981) Mutants of satellite Virus P4 that cannot derepress their bacteriophage P2 helper. J Mol Biol 148:1–19
Ghisotti G, Zangrossi S, Sironi G (1983) An Escherichia coli gene required for P2-λ interference. J Virol 48:616–626
Goldstein R, Sedivy J, Ljungquist E (1982) Propagation of satellite phage P4 as a plasmid. Proc Natl Acad Sci USA 79:515–519
Kahn M, Ow D, Sauer B, Rabinowitz A, Calendar R (1980) Genetic analysis of bacteriophage P4 using P4-plasmid Col E1 hybrids. Mol Gen Genet 177:399–412
Laemmli U (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lagos R, Goldstein R (1984) Plasmid P4: manipulation of plasmid copy number and induction from the integrated state. J Bacteriol 158:208–215
Lin CS (1984) Nucleotide sequence of the essential region of bacteriophage P4. Nucleic Acids Res 12:8667–8684
Lindqvist BH, Six EW (1971) Replication of bacteriophage P4 DNA in a nonlysogenic host. Virology 43:1–7
Ow D, Ausubel A (1980) Recombinant P4 bacteriophages propagate as viable lytic phages or as autonomous plasmids in Klebsiella pneumoniae. Mol Gen Genet 180:165–175
Sancar A, Hack AM, Rupp WD (1979) Simple method for identification of plasmid-coded proteins. J Bacteriol 137:692–693
Sancar A, Wharton RP, Seltzer S, Kacinski B, Clarke ND, Rupp WD (1981) Identification of uvr A gene product. J Mol Biol 148:45–62
Shore D, Dehò G, Tsipis J, Goldstein R (1978) Determination of capsid size by satellite bacteriohage P4. Proc Natl Acad Sci USA 75:400–404
Six EW, Klug CA (1973) Bacteriophage P4: a satellite virus depending on a helper such as prophage P2. Virology 51:327–344
Six EW, Lindqvist BH (1978) Mutual derepression in the P2–P4 bacteriophage system. Virology 87:217–230
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Communicated by R. Devoret
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Alano, P., Dehò, G., Sironi, G. et al. Regulation of the plasmid state of the genetic element P4. Mol Gen Genet 203, 445–450 (1986). https://doi.org/10.1007/BF00422069
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DOI: https://doi.org/10.1007/BF00422069